Table 1 Measurements and Adjustments of physiological parameters in the isolated pig heart model
Parameter | Assessed by | Controlled by | Experimental setup |
|---|---|---|---|
pO2, pCO2 | Oxygen probe (interstitial) Part. Pressure (ven., art.) | Blood oxygenator, oxygenation of dialysate | Levels of pO2: fully adjustable to induce graded hypoxia Levels of pCo2: Adjusted to pH |
pH | blood gas analysis | CO2 saturation | Constant |
Preload, Afterload | Tip Manometer | Adjustable | |
CPP, CBF | Tip Manometer, Flowmeter | Flow/Afterload | individually for each coronary |
Heart rate | ECG | Pacer | permanent registration |
LVP, RVP | conductance catheter, tip-manometer | Constant (Modified according to the afterload level) | |
Troponin/Myoglobin/Creatine kinase/glucose/lactate | blood samples | Blood Gas Analysis | |
Tissue | EMB (Histology, immunohisto-chemistry, electron microscopy) | ||
(CPP Coronary perfusion pressure, LVP left ventricular pressure, RVP right ventricular pressure, SV stroke volume, dP/dt pressure over time/myocardial contractile function, DTF diastolic time frame, EMB endomyocardial biopsy) |