Volume 12 Supplement 1

Abstracts of the 13th Annual SCMR Scientific Sessions - 2010

Open Access

Results of a tissue engineered pulmonary valve in humans assessed with CMR

  • Carsten Rickers1,
  • Andreas Entenmann1, 2,
  • Gunther Fischer1,
  • Jens Scheewe1,
  • Michael Scheid1,
  • Michael Jerosch-Herold3,
  • Jan Braesen1,
  • Inga Voges1,
  • Chris Hart1 and
  • Hans-Heiner Kramer1
Journal of Cardiovascular Magnetic Resonance201012(Suppl 1):P17

DOI: 10.1186/1532-429X-12-S1-P17

Published: 21 January 2010

Introduction

Tissue-engineered (TEng) valves have recently been developed as an alternative to pulmonary valve replacement with homograft conduits to improve device life-time and function.

Purpose

To evaluate with advanced CMR techniques the morphological and functional properties of a decellularized porcine xenograft valve conduit (Matrix-P™) and its successor product with additional equine pericardium sheathing (Matrix-P-Plus™; AutoTissue, Germany).

Methods

TEng valves had been implanted into the right ventricular outflow tract of 12 pts (age: 15 ± 8,5 yrs; Matrix-P™ n = 7; Matrix-P-Plus™ n = 5) due to pulmonary stenosis or insufficiency within a median 5 months range (2-7 months) before a comprehensive follow-up by CMR. Echocardiography was suspicious of accelerated blood flow in the main pulmonary artery in 10 of those. The severity of the pulmonary valve stenosis was assessed by gradient echo cine MRI (FOV 270 × 270 mm, voxel size 1.88 × 1.94 × 5 mm, TR/TE/α = 4.4 ms/2.7 ms/15°) and phase-contrast velocity mapping (FOV 270 × 270 mm, voxel size 1.64 × 1.4 × 7 mm, TR/TE/α = 15 ms/2.5 ms/30°, velocity encoding (VENC) = 400 cm/s) were applied. For tissue characterisation late enhancement imaging (Gd 0.1 mmol/kg) using an ECG triggered 3D inversion recovery sequence (TR/TE/α= 3.7 ms/1.8 ms/15°; voxel size 1.17 × 1.27 × 10 mm) and T1 and T2 weighted turbo spin echo sequences were utilized.

Results

In 9 pts (33%) CMR detected a significant conduit stenosis (Vmax 3.7 ± 1,3 m/s) with mild or moderate insufficiency (RGF 21.3 ± 5.9%) post-surgery. In 3 pts the conduit was considered to be normal. In all pts with a stenosis, T1-weighted images showed significant paravalvular Gadolinium enhancement and wall thickening of the conduit. In 7 pts the "Matrix" valve conduit was replaced by a homograft. Explants were evaluated through histological analysis that showed granulomatous inflammation and increased fibrous tissue content correlating with CMR findings.

Conclusion

Conduit malfunction and paravalvular inflammation was successfully detected with CMR in pts post implantation of a decellularized pulmonary xenograft. As a consequence of the devastating short term results, use of these grafts was terminated at our center after initial use in 27 patients.

Authors’ Affiliations

(1)
University Hospital Schleswig-Holstein
(2)
Universtiy Hospital Kiel
(3)
Department of Radiology, Brigham & Women's Hospital & Harvard Medical School

Copyright

© Rickers et al; licensee BioMed Central Ltd. 2010

This article is published under license to BioMed Central Ltd.

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