Comparison of long and short axis quantification of left ventricular volume parameters by cardiovascular magnetic resonance, with ex-vivo validation
- Helene Childs†1,
- Lucia Ma†1,
- Michael Ma1,
- James Clarke2,
- Myra Cocker1,
- Jordin Green1, 3,
- Oliver Strohm1 and
- Matthias G Friedrich1Email author
© Childs et al; licensee BioMed Central Ltd. 2011
Received: 12 April 2011
Accepted: 11 August 2011
Published: 11 August 2011
The purpose of the study was to compare the accuracy and evaluation time of quantifying left ventricular (LV), left atrial (LA) volume and LV mass using short axis (SAX) and long axis (LAX) methods when using cardiovascular magnetic resonance (CMR).
Materials and methods
We studied 12 explanted canine hearts and 46 patients referred for CMR (29 male, age 47 ± 18 years) in a clinical 1.5 T CMR system, using standard cine sequences. In standard short axis stacks of various slice thickness values in dogs and 8 mm slice thickness (gap 2 mm) in patients, we measured LV volumes using reference slices in a perpendicular, long axis orientation using certified software. Volumes and mass were also measured in six radial long axis (LAX) views.
LV parameters were also assessed for intra- and inter-observer variability. In 24 patients, we also analyzed reproducibility and evaluation time of two very experienced (> 10 years of CMR reading) readers for SAX and LAX.
In the explanted dog hearts, there was excellent agreement between ex vivo data and LV mass and volume data as measured by all methods for both, LAX (r2 = 0.98) and SAX (r2 = 0.88 to 0.98). LA volumes, however, were underestimated by 13% using the LAX views. In patients, there was a good correlation between all three assessed methods (r2 ≥ 0.95 for all). In experienced clinical readers, left-ventricular volumes and ejection fraction as measured in LAX views showed a better inter-observer reproducibility and a 27% shorter evaluation time.
When compared to an ex vivo standard, both, short axis and long axis techniques are highly accurate for the quantification of left ventricular volumes and mass. In clinical settings, however, the long axis approach may be more reproducible and more time-efficient. Therefore, the rotational long axis approach is a viable alternative for the clinical assessment of cardiac volumes, function and mass.
Cardiovascular magnetic resonance imaging (CMR) is well recognized as a gold standard for functional imaging and assessment. CMR using state-of-the-art sequences has been shown to be very reproducible, is considered appropriate for many clinical indications  and there is consensus about its clinical use and methodology ; newer sequences, however, have not been evaluated against an ex vivo gold standard. Furthermore, short axis (SAX) stacks, considered the standard approach for quantifying left ventricular (LV) volumes and function and used as such for validation , are time consuming and there are partial volume effects leading to problems that arise upon defining the slice area in the most basal and most apical slices . Inclusion or exclusion of basal slices is of particular interest since until now, the blood volume in the LV outflow tract is often excluded, while atrial volume may be erroneously included. Likely due to this problem, the utilization of SAX views may be hampered by a lesser reproducibility .
Long axis views (LAX) have been proposed as an alternative to SAX-derived methods . We aimed to validate state-of-the-art sequences in SAX and rotational LAX views in explanted canine hearts and to compare the two methods in clinical CMR studies. This is of clinical importance for LV function and mass analysis, but also for correlating these results with tissue abnormalities such as high signal intensity areas in late Gd enhancement or T2-weighted images.
Materials and methods
All examinations were performed on a 1.5 T system (MAGNETOM Avanto®, Siemens Healthcare, Erlangen, Germany). All quantitative CMR parameters were assessed using certified software (cmr42, Circle Cardiovascular Imaging Inc., Calgary, Canada). All readers had training on drawing contours with special emphasis on how to avoid misinterpretation of the basal slice.
Ex vivo studies
We assessed 46 individuals, (29 male, 17 female; mean age 47 ± 18, range 15-79 years). 4 were healthy volunteers without evidence for heart disease, while 42 patients were randomly selected from clinical referrals for functional imaging, tissue characterisation and/or viability. The study was approved by the local ethics committee and informed consent was obtained from each individual.
Standard Steady-State-Free-Precession (SSFP) cine sequences were used for all approaches. Typical parameters were: TE 1.1 ms, TR 2.31 ms, FA 75°, matrix 340 × 284 mm, pixel size 1.3 mm × 1.4 mm and an IPAT factor 2. With 25 lines per segment, the effective repetition time typically was 57 ms. SAX acquisition was performed as multiple short axes across the entire LV in an imaging plane perpendicular to the LAX of the LV with a slice thickness of 8 mm and a 2 mm gap. The LAX slices were performed using 6 slices rotating in 30 degree increments around the anatomical LV LAX.
CMR image analysis was performed using certified software (cmr42, Circle Cardiovascular Imaging Inc., Calgary, Canada) by well-trained observers. Endocardial and epicardial contours were drawn manually for the LV at end-systole and end-diastole in each data set.
We assessed intra-observer variability by having one observer analyzing volumes with SAX twice, while a different observer analyzed volumes with LAX approach twice. To minimize observer bias, each of the two less experienced readers read only one of the orientations, either SAX or LAX. This may have introduced a bias for the time needed. Both readers, however, had similar training before the study. Furthermore, for experienced readers such a bias can be excluded, since they read both, SAX and LAX view in separate sessions.
For all methods, end-diastolic volume (EDV), end-systolic (ESV), stroke volume (SV), ejection fraction (EF), and LV mass were assessed by three independent observers. LV mass was measured at end-systole by both methods because of the easier definition of the endocardial borders in the contracted myocardium. The time needed to complete assessments was recorded in a randomly selected subgroup of 12 individuals.
Explanted canine hearts
Differences between CMR measurements and actual results with correlation coefficients in explanted canine hearts
SAX 10 mm
SAX 8 mm
SAX 5 mm
23.3 ± 5.3 ml
-2.5 ± 1.1%
CV = 1.18
-7.0 ± 7.8%
CV = 3.63
-0.7 ± 4.6%
CV = -12.1
+0.3 ± 5.3%
CV = -12.7
11.8 ± 7.5 ml
-13.3 ± 7.0%
CV = 1.61
-4.2 ± 6.9%
CV = 3.49
-3.3 ± 6.9%
CV = 6.07
+5.3 ± 9.1%
CV = -9.24
99.6 ± 5.5 g
-3.3 ± 3.0%
CV = 2.68
+7.5 ± 5.6%
CV = -3.49
+4.0 ± 4.2%
CV = -5.31
+0.1 ± 6.0%
CV = 17.3
Seventeen of the 42 patients referred for CMR did not reveal any abnormalities, while 25 had various cardiac conditions. Image quality was good in all cases, none of the patients were excluded from the analysis.
Volumetric results as calculated from the different analysis methods in patients
Short axis views
Long axis views
177 ± 99 ml
182 ± 101 ml
94 ± 102 ml
91 ± 97 ml
55 ± 16%
55 ± 15%
144 ± 64 g
141 ± 62 g
Absolute inter-observer differences (%) using different acquisition orientations in patients.
Short axis views
Long axis views
11.2 ± 7.9 ml
4.4 ± 4.6 ml *
18.6 ± 12.5 ml
7.0 ± 5.1 ml *
7.2 ± 5.7%
3.0 ± 1.7%*
14.0 ± 14.4 g
7.1 ± 6.8 g
Time needed for evaluation using different acquisition orientations in patients.
Short axis views
Long axis views
8:42 ± 4:38
6:24 ± 0:49*
Less experienced reader
13:08 ± 7:14
12:42 ± 3:34
Our data indicate that both, cross-referenced short axis and long axis views provide accurate measurements of volumetric LV parameters. This is not specific for CMR and has implications for other imaging techniques such as CT. Furthermore, our data indicate that long axis views take less time for evaluation. Another advantage of long axis views is the low susceptibility to errors induced by misinterpretation of slices close to the mitral valve. In fact, a post-hoc analysis of our data showed that errors of standard short axis data were due to inclusion or exclusion of the most basal slice, while there was no difference between cross-referenced and long axis results. Therefore, long axis planes may be a useful approach in most routine clinical situations.
LV mass was larger when calculated by SAX. This could be due to the inclusion of trabeculations and papillary muscles in SAX while only papillary muscles were included in LAX; however, we were concerned that including both trabeculations and papillary muscles in the LAX would lead to an over-estimation in LV mass due to the use of rotational volumetry calculation . Another possible explanation is that SAX images experience partial volume effects in basal and apical slices while in radial LAX images the identification of subendocardial borders is easier.
Most validation studies have been performed using SAX measurements of LV mass [8–10]. Furthermore, tissue characterization often is performed in SAX views and therefore this view may be better for correlating results between sequences. Since abnormalities seen in images used for tissue characterization should be verified by cross-sectional views and therefore both long and short axis views are required anyway, a comparison of tissue characteristics with the cine images is equally possible. More recent studies indicate that long-axis based methods may be equally accurate yet may have a smaller intra-observer variability . This may be due to the clear visualisation of both mitral and aortic valve planes and the reduction of partial volume effects allowing for well-defined myocardial boundaries . So, while LAX methods use geometric assumptions, the advantage of having a perpendicular view on the basal and apical borders of the left ventricle may outweigh the disadvantage of partially replacing actual measurements by computed data.
We did not systematically assess the impact of ventricular shape criteria on our results. Although this has not been studied systematically, a highly irregular morphology may limit the accuracy of LAX data . In the LAX views, traceless and papillary muscles were only partially included into LV mass, whereas they were completely included in the SAX stack evaluation. This is due to the fact, that inclusion of small structures (i.e. smaller than the distance tracked by the 30° rotation between LAX planes) leads to overestimation of the volume of these tissues (unpublished data). This inconsistency between the methods however was not associated with significant differences between methods; yet, it may explain the non-significant trend toward underestimation of LV mass by LAX. For clinical scenarios, the lack of significant differences let this problem appear irrelevant. In the SAX method, we used no gaps for the dog studies, but had gaps in patients. The reason for using no gaps in dogs was simply to compare the results by both methods excluding other confounders. Resembling clinical scenarios, however, we applied gaps in patient studies as typically done in clinical applications and clinical research . The applicability of our results to other readers may be limited by individual approaches how to deal with inclusion or exclusion of papillary muscles and trabecular tissue.
When compared to an ex vivo standard, both, short axis and long axis techniques are highly accurate for the quantification of left ventricular volumes and mass. In patients, however, the long axis approach may be more reproducible and more time-efficient. For the analysis of left ventricular volumes by tomographic techniques, mass and function in hearts without severe shape alterations, a long axis approach may be a viable alternative
We are thankful for the wonderful assistance provided by Loreen Thon and Sheri-Lee Rinella. We also want to thank the laboratory of Dr. John Tyberg for providing the hearts.
- Hendel RC, Patel MR, Kramer CM, Poon M, Hendel RC, Carr JC, Gerstad NA, Gillam LD, Hodgson JM, Kim RJ, Kramer CM, Lesser JR, Martin ET, Messer JV, Redberg RF, Rubin GD, Rumsfeld JS, Taylor AJ, Weigold WG, Woodard PK, Brindis RG, Hendel RC, Douglas PS, Peterson ED, Wolk MJ, Allen JM, Patel MR: ACCF/ACR/SCCT/SCMR/ASNC/NASCI/SCAI/SIR 2006 appropriateness criteria for cardiac computed tomography and cardiac magnetic resonance imaging: a report of the American College of Cardiology Foundation Quality Strategic Directions Committee Appropriateness Criteria Working Group, American College of Radiology, Society of Cardiovascular Computed Tomography, Society for Cardiovascular Magnetic Resonance, American Society of Nuclear Cardiology, North American Society for Cardiac Imaging, Society for Cardiovascular Angiography and Interventions, and Society of Interventional Radiology. J Am Coll Cardiol. 2006, 48: 1475-97. 10.1016/j.jacc.2006.07.003.View ArticlePubMedGoogle Scholar
- Hundley WG, Bluemke DA, Finn JP, Flamm SD, Fogel MA, Friedrich MG, Ho VB, Jerosch-Herold M, Kramer CM, Manning WJ, Patel M, Pohost GM, Stillman AE, White RD, Woodard PK: ACCF/ACR/AHA/NASCI/SCMR 2010 Expert Consensus Document on Cardiovascular Magnetic Resonance: A Report of the American College of Cardiology Foundation Task Force on Expert Consensus Documents. Circulation. 2010, 121: 2462-508.View ArticlePubMedGoogle Scholar
- François CJ, Fieno DS, Shors SM, Finn JP: Left ventricular mass: manual and automatic segmentation of true FISP and FLASH cine MR images in dogs and pigs. Radiology. 2004, 230: 389-95. 10.1148/radiol.2302020761.View ArticlePubMedGoogle Scholar
- Dulce MC, Mostbeck GH, Friese KK, Caputo GR, Higgins CB: Quantification of the left ventricular volumes and function with cine MR imaging: comparison of geometric models with three-dimensional data. Radiology. 1993, 188: 371-6.View ArticlePubMedGoogle Scholar
- Bloomer TN, Plein S, Radjenovic A, Higgins DM, Jones TR, Ridgway JP: Sivananthan MU Cine MRI using steady state free precession in the radial long axis orientation is a fast accurate method for obtaining volumetric data of the left ventricle. J Magn Reson Imaging. 2001, 14: 685-92. 10.1002/jmri.10019.View ArticlePubMedGoogle Scholar
- Bloomgarden DC, Fayad ZA, Ferrari VA, Chin B, Sutton MG, Axel L: Global cardiac function using fast breath-hold MRI: validation of new acquisition and analysis techniques. Magn Reson Med. 1997, 37: 683-92. 10.1002/mrm.1910370510.View ArticlePubMedGoogle Scholar
- Papavassiliu T, Kühl HP, Schröder M, Süselbeck T, Bondarenko O, Böhm CK, Beek A, Hofman MM, van Rossum AC: Effect of endocardial trabeculae on left ventricular measurements and measurement reproducibility at cardiovascular MR imaging. Radiology. 2005, 236: 57-64. 10.1148/radiol.2353040601.View ArticlePubMedGoogle Scholar
- Keller AM, Peshock RM, Malloy CR, Buja LM, Nunnally R, Parkey RW, Willerson JT: In vivo measurement of myocardial mass using nuclear magnetic resonance imaging. J Am Coll Cardiol. 1986, 8: 113-7. 10.1016/S0735-1097(86)80100-0.View ArticlePubMedGoogle Scholar
- Shapiro MD, Nieman K, Nasir K, Nomura CH, Sarwar A, Ferencik M, Abbara S, Hoffmann U, Gold HK, Jang IK, Brady TJ, Cury RC: Utility of Cardiovascular Magnetic Resonance to Predict Left Ventricular Recovery After Primary Percutaneous Coronary Intervention for Patients Presenting With Acute ST-Segment Elevation Myocardial Infarction. The American Journal of Cardiology. 2007, 100: 211-6. 10.1016/j.amjcard.2007.02.079.View ArticlePubMedGoogle Scholar
- Katz J, Milliken MC, Stray-Gundersen J, Buja LM, Parkey RW, Mitchell JH, Peshock RM: Estimation of human myocardial mass with MR imaging. Radiology. 1988, 169: 495-8.View ArticlePubMedGoogle Scholar
- Friedrich MG, Schulz-Menger J, Strohm O, Dick AJ, Dietz R: The diagnostic impact of 2D- versus 3D left ventricular volumetry by MRI in patients with suspected heart failure. Magma. 2000, 11: 16-9. 10.1007/BF02678483.View ArticlePubMedGoogle Scholar
- Hudsmith LE, Petersen SE, Francis JM, Robson MD, Neubauer S: Normal human left and right ventricular and left atrial dimensions using steady state free precession magnetic resonance imaging. J Cardiovasc Magn Reson. 2005, 7: 775-82. 10.1080/10976640500295516.View ArticlePubMedGoogle Scholar
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