Methodologies used to acquire superficial femoral artery blood flow and mid-thigh muscle and fat composition. The location for measurement of flow within the arteries of interest was in the axial plane 20 cm distal to femoral head. The magnified images at the top right demonstrate the cross-section of superficial femoral artery used to measure flow. The images at the bottom demonstrate the mid-thigh cross-sections acquired just distal to the superficial femoral arterial flow images. As shown, color-coded signal thresholding was used to identify fat and skeletal muscle in the middle thigh.