Figure 1
From: Viability imaging of stem cell using a MRI reporter gene and MEMRI
Functional confirmation of the MRI RG and MRI and BLI. A In vitro of hESC-RD with SPIO-conjugated anti-c-myc and HA antibody showed strong T2 weighted dephaing signal (c-d). Non-transduced mESCs showed no T2 weighted dephasing signal (a-b). MR image was taken with GRE sequence using the following parameters: 100 ms TR, 30 ms TE, FA 45, FOV 12, NEX1, 192 × 192. B. Long axis view of mouse heart after injection of 0.5 × 106 magnetically pre-labeled hESC onto the lateral left ventricular wall. C. Validation of viability of hESC using bioluminescence imaging (BLI). D. MEMRI of transplanted mESC-RG on bilateral mouse hindlimbs (indicated by white arrows). E. Viability of the injected mESC was confirmed with positive luciferase activity using BLI.