Skip to main content
Figure 2 | Journal of Cardiovascular Magnetic Resonance

Figure 2

From: Functionalization of gadolinium metallofullerenes for detecting atherosclerotic plaque lesions by cardiovascular magnetic resonance

Figure 2

A. Upregulation of CD36 receptors on foam cells. U937 cells (in duplicate) were treated as described in Methods, washed and incubated with antibodies to CD36 or an isotype matched control. The graph shows the average mean fluorescent intensity (MFI) of the signal (±SD) and is representative of three separate experiments. B. Testing ATCA for foam cell binding. U937 cells (non-foam) were converted into foam cells (foam) using oxLDL and PMA as described. Cells were washed and ATCA (10 μg/ml) added overnight in a CO2 incubator. The next day cells were washed with media and the supernatants (S) and pellets (P) subjected to ICP for Gd detection. The percentage of Gd in the pellet and supernatant was calculated based on the total amount added to the cells.

Back to article page