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Figure 6 | Journal of Cardiovascular Magnetic Resonance

Figure 6

From: At-risk but viable myocardium in a large animal model of non ST-segment elevation acute coronary syndrome: cardiovascular magnetic resonance with ex vivo validation

Figure 6

Microscopy. Top) Representative light photomicrographs from animals in the sham (A) vs. NSTE-ACS model (B) groups. H&E staining revealed no significant contraction band necrosis to indicate irreversible cell death in either group. Middle) Representative electron photomicrographs of left ventricular myocardial tissue (noting the mitochondria – arrows) corresponding to the remote region from a representative sham animal (C, white), the matching affected region from the same sham animal (D, green), the remote region from a representative animal from the model group (E, white) and the matching affected region from the same model animal (F, green) (Original magnification: 11,000×; Staining: 2% uranyl acetate and Reynolds lead citrate). Bottom) Electron microscopy showed no evidence of mitochondrial damage (e.g., decreased matrix density, disintegration of cristae) or structural signs of irreversible muscle damage. Although mitochondria were noted to be consistently larger in the lateral LV wall compared to the anterior LV wall in controls and NSTE-ACS model animals, there was no objective evidence of mitochondrial swelling, as reflected by computer-derived measures of mitochondrial transectional area, perimeter and aspect ratio, in the NSTE-ACS group. Data presented as ratios of measurements made on mitochondria within the affected region relative to those made on corresponding mitochondria within the remote region in the same animal.

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