T2* method and inter-observer reproducibility. (A) A transverse slice of the liver where an example of the region of interest (ROI) is seen in green within the mid/lateral area free of large vessels. (B) T2* estimation using the truncation method in exponential curve fitting: crosses represent truncated data points whereby only the first 5 points contribute to the decay curve model. (C) The inter-observer reproducibility and agreement for R2* between observer 1 and 2 (both in triplicate, error bars show SEM, 96 scans) with line of identity. Coefficient of variation 5.79%. (D) Bland-Altman analysis of inter-observer liver R2* percentage differences plotted against the mean R2* of the two observers. The bias as the mean of all differences was 0.61 ± 4.29% (SD). The 95% limits of agreement ( LoA) were between -7.79 and 9.02%.