Representative adenosine stress/rest myocardial perfusion images in a HFHS pig without obstructive CAD and expected to have coronary microvascular dysfunction (CMD), demonstrating the ability to visually detect diffuse vasodilator-induced hypoperfusion at the subendocardial layer. (a,b): End-systolic adenosine stress/rest first-pass perfusion images with a prescribed 1.2 × 1.2 mm in-plane resolution corresponding to the peak myocardial-enhancement phase (no image interpolation used). The self-gated steady-state imaging approach enables reconstruction of all myocardial slices at the end-systolic phase. Stress-induced subendocardial perfusion defects are diffusely present in all myocardial slices and vessel territories, consistent with CMD. (c): Zoomed-in stress image (mid slice) shows the highly-resolved myocardium with multiple "defect pixels" present across the subendocardium: the measured endocardial-epicardial distance is nearly 10 times the size of a single image pixel.