Results for quantitative assessment of myocardial perfusion gradients using the previously established transmural perfusion gradient (TPG) analysis method. (a): Comparison of slice-averaged mean TPG (normalized value in percentage) for the HFHS versus control pigs, showing a significantly higher TPG value for the HFHS group in comparison to controls (24% ± 5% vs 5% ± 3%). (b): Bull's eye map of mean TPG (normalized value for each of the 16 myocardial segments) averaged across the n = 4 pigs in each group. The TPG analysis results are consistent with the visual assessment (example shown in Fig. 1) and demonstrate a marked stress-induced transmural gradient in the HFHS pigs compared with controls.