Table 1 List of primary and secondary antibodies utilized for western immunoblotting experiments

Primary antibodies (Cat #)

Source

Supplier

Dilution

Application(s)

 Akt (9272)

Rab PC

Cell Signaling Technol.

1:1000

WB

 P-Akt (9271) (Ser473)

Rab PC

Cell Signaling Technol.

1:1000

WB

 ß-Actin

Mouse MC

Sigma-Aldrich

1:5000

WB

Secondary antibodies (Cat #)

Source

Supplier

Dilution

Application(s)

 Goat Anti-Mouse IgG-HRP Conjugate (170-6516)

Goat

BioRad

1:5000

WB

 Anti-Rab IgG-HRP conjugate (170-6515)

Goat

BioRad

1:10000

WB