Fig. 1

Flowchart of mice in different diet groups and treatment. HFHSD mice (C57BL6J on High fat high sucrose diet) and SD mice (C57BL6J on control diet) were kept on diet for 16 weeks in the first experiment (glucose homeostasis study), both glucose and insulin tolerance tests were performed every month in order to determine time of impaired glucose homeostasis. In the second experiment (chronological study), a magnetic resonance (MR) examination was performed every month and intraperitoneal glucose tolerance test was done at the end of experiment (16 weeks) before sacrifice. In the third experiment (experimental study), SD mice were kept on standard diet only for 16 weeks until MR examination and IPGTT. Mice fed HFHSD underwent MR examination and IPGTT at 16 weeks, before treatment with exendin-4 or physiological serum during 14 days and then underwent once again MR examination and IPGTT after treatment