Fig. 2

Characterization of HFHSD-induced obesity model. Mice fed the HFHSD became significantly obese (a) and had an increased abdominal fat volume at the first time point (b). Time course of intraperitoneal insulin tolerance test revealed significantly impaired glucose tolerance (c) and higher fasting plasma glycemia (d) in the HFHSD group at 2 months post-diet. Examples of intraperitoneal glucose test tolerance at 1-month (glucose administration arrow, 1 g/kg) (e), and intraperitoneal insulin tolerance test (insulin administration arrow, 0.75 mU/g) at 1 months (f) and 2-month (g) post diet. Data are means ± SEM. Two-way Anova test with repeated measures has been performed to assess differences *P < 0,05; **p < 0,005; ***p < 0,0005 vs SD