Fig. 2

Investigation of segmental and transmural myocardial iron distribution. The photograph shows a mid-myocardial cut slice from the formalin-fixed heart of pig 8. The slice was cut into 6 segments: Anteroseptal (1), inferolateral (2), inferior (3), inferoseptal (4), anterolateral (5) and anterior (6). Each segment was divided into an epicardial-, mesocardial and endocardial layer (as indicated for segment 3), leading to 18 tissue samples from each heart for chemical cardiac iron concentration (CIC) assessment. Epicardial layers of the right ventricular septum (segment 1 and 2) represent endocardium