Cardiac structure and function during ageing in energetically compromised Guanidinoacetate N-methyltransferase (GAMT)-knockout mice – a one year longitudinal MRI study

Table 2 Summary of the significant findings from the mixed-effects regression

Trait ^a	Factor ^b	Coefficient estimate	95% CI	P-value
BW	Sex	-5.13	[-6.72, -3.54]	<0.0001
	Genotype	-2.40	[-4.3, -0.49]	<0.0001
	Time	-	-	<0.0001
	4 months	6.34	[4.86, 7.82]	<0.0001
	8 months	8.28	[6.9, 9.66]	<0.0001
	12 months	11.73	[10.33, 13.13]	<0.0001
	Genotype*Time	-	-	0.0003
	Genotype*4 months	-1.52	[-3.55, 0.52]	0.1415
	Genotype*8 months	-2.60	[-4.56, -0.64]	0.0102
	Genotype*12 months	-4.79	[-6.88, -2.69]	<0.0001
LVM	BW	0.88	[-0.06, 1.81]	<0.0001
	Sex	-4.50	[-13.36, 4.35]	0.2636
	Genotype	-8.85	[-16.91, -0.78]	0.7443
	Time	-	-	0.0003
	4 months	15.35	[7.9, 22.8]	0.0001
	8 months	18.55	[10.27, 26.84]	<0.0001
	12 months	19.29	[8.84, 29.74]	0.0005
EF	Sex	1.06	[-2.55, 4.66]	0.4924
	Genotype	3.46	[-0.02, 6.94]	0.0334
	Time	-	-	0.0027
	4 months	-1.43	[-5.58, 2.72]	0.4947
	8 months	-5.42	[-9.44, -1.4]	0.009
	12 months	-7.30	[-11.55, -3.06]	0.001
Heart rate	Sex	-48.49	[-74.64, -22.33]	0.1958
	Genotype	-42.08	[-67.96, -16.2]	0.698
	Time	-	-	0.0519
	4 months	4.99	[-17.61, 27.58]	0.6611
	8 months	-23.16	[-44.79, -1.54]	0.0362
	12 months	-20.67	[-43.49, 2.15]	0.0751
	Sex*Genotype	73.26	[35.93, 110.6]	0.0004
dV/dt_max.EDV^-1	Sex	0.002	[0.001, 0.004]	0.0048
	Genotype	0.000	[-0.001, 0.002]	0.8454
	Time			0.0009
	4 months	-0.001	[-0.003, 0.001]	0.2688
	8 months	-0.003	[-0.005, -0.001]	0.0017
	12 months	-0.004	[-0.005, -0.002]	0.0004

^a Traits for which at least one factor in the model had significant (P < 0.01) effects.
^b Estimated effects of KO compared to WT (baseline) for Genotype, female compared to male (baseline) for Sex, and trait values at 4 months, 8 months, and 12 months compared to trait values at 6 weeks (baseline) for Time (3df test)

ISSN: 1532-429X