Absence of DNA double strand breaks in human peripheral blood mononuclear cells after magnetic resonance imaging assessed by γH2AX flow cytometry: a prospective blinded trial
Journal of Cardiovascular Magnetic Resonance volume 18, Article number: O129 (2016)
Magnetic Resonance Imaging (MRI) is regarded as a non harming and non invasive imaging modality with superb tissue contrast and almost no side effects. Compared to other cross-sectional imaging modalities MRI does not use ionizing radiation. Recently however, strong magnetic fields as applied in clinical MRI scanners have been suspected to induce DNA double strand breaks in human lymphocytes.
In this study we investigated the impact of 3 T cardiac MRI examinations on the induction of DNA double strand breaks in peripheral mononuclear cells by ɣH2AX staining and flow cytometry analysis. The study cohort consisted of 73 healthy non-smoking volunteers with 36 volunteers undergoing CMRI and 37 controls without intervention. Differences between the two cohorts were analysed by a mixed linear model with repeated measures.
Both cohorts showed a significant increase of the ɣH2AX signal from baseline to post procedure of 6·7% and 7·8%, respectively. The difference between the two groups was not significant.
We therefore conclude that clinical 3 T MRI as used in cardiac imaging does not have an impact on DNA integrity.
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Fasshauer, M., Krüwel, T., Zapf, A. et al. Absence of DNA double strand breaks in human peripheral blood mononuclear cells after magnetic resonance imaging assessed by γH2AX flow cytometry: a prospective blinded trial. J Cardiovasc Magn Reson 18 (Suppl 1), O129 (2016). https://doi.org/10.1186/1532-429X-18-S1-O129