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Volume 18 Supplement 1

19th Annual SCMR Scientific Sessions

Cardiovascular magnetic resonance imaging produces highly reproducible rodent cardiac volumetric and functional data using a 1.5 Tesla scanner

Background

Rodent models of cardiovascular disease provide a means of rapid assessment of disease processes and therapeutic interventions. Cardiac magnetic resonance (CMR) provides superior functional imaging over other modalities, however has technical challenges in rodents due to small hearts sizes and rapid heart rates. High field strength scanners are often required, however access is limited. We therefore aimed to determine the reproducibility of functional CMR imaging of rodents using a clinical scanner.

Methods

Eight healthy Lewis rats underwent CMR imaging using a 1.5T scanner and a clinical wrist coil. Electrocardiograph-gated bSSFP cine imaging was used to acquire ventricular long axis, four chamber and short-axis views. End-diastolic volume (EDV), ejection fraction (EF) and myocardial mass (MM) were measured using short-axis stack images. After initial scanning, the rodents were taken off the scanner table, then immediately returned for repeat imaging. Intra- and inter-observer variability and inter-study reproducibility were assessed. Sample size calculations were performed to determine animal numbers needed to detect a 10% difference in cardiac volumes, EF or MM. Comparison was made with two-dimensional echocardiography using published data.

Results

Image quality was invariably good (Figure). Excellent intra-observer reproducibility was observed: coefficients of variability (COV) 3.17%, 1.61% and 5.39% for EDV, EF and MM. Inter-observer variability was likewise good: COV were 7.88%, 5.88% and 10.91% respectively. Inter-study reproducibility was likewise high: COV 6.93%, 4.56% and 3.66% respectively. Measurement agreements were favourable compared to rodent CMR studies using high field-strength scanners and human CMR reproducibility studies. Measurement agreements were superior to rodent and human echocardiography. A calculated sample size reduction of 96% can be achieved to detect a 10% change in MM using 1.5T CMR compared with two-dimensional echocardiography, with reductions in sample sizes by 65% and 78% to detect a 10% change in EDV and EF respectively.

Conclusions

Accurate volumetric and functional data from healthy rodent hearts are obtainable using a clinical 1.5T scanner. The application of this protocol will allow wider access to CMR imaging in small rodent cardiovascular disease and therapeutic models with significant reduction in sample size to allow more accurate volumetric and functional measurements compared to echocardiography.

Figure 1
figure1

Images of rat hearts in end-diastole and end-systole in ventricular four chamber (a and b), two chamber (c and d) and mid-ventricular short axis (e and f) views.

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Correspondence to Gayathri Kumarasinghe.

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This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Kumarasinghe, G., Yu, C.Y., Chang, M. et al. Cardiovascular magnetic resonance imaging produces highly reproducible rodent cardiac volumetric and functional data using a 1.5 Tesla scanner. J Cardiovasc Magn Reson 18, P191 (2016). https://doi.org/10.1186/1532-429X-18-S1-P191

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Keywords

  • Cardiovascular Magnetic Resonance
  • Cardiac Magnetic Resonance
  • Cardiac Magnetic Resonance Imaging
  • Cardiovascular Magnetic Resonance Imaging
  • Myocardial Mass