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Volume 18 Supplement 1

19th Annual SCMR Scientific Sessions

  • Walking poster presentation
  • Open Access

Correlations and validations of dual-bolus and dual-sequence quantification of first-pass myocardial perfusion CMR in humans and canines

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Journal of Cardiovascular Magnetic Resonance201618 (Suppl 1) :Q17

  • Published:


  • Myocardial Blood Flow
  • Arterial Input Function
  • Saturation Recovery
  • Time Intensity Curve
  • Composite Pulse


Dual-bolus and dual-sequence techniques have been proposed to maintain the linearity of arterial input function (AIF) in LV during first-pass CMR perfusion imaging. This study compared myocardial blood flow (MBF) estimates using both techniques in humans and in a canine model.


CMR perfusion imaging was performed in six canines and thirty patients at 1.5T using dual-bolus (0.005 and 0.05 mmol/kg Gd-DTPA) and dual-sequence techniques with 1RR, 90° composite pulse, 50° SSFP readout, saturation recovery 90 ms, TR 2.4 ms, TE 1.2 ms, matrix size 128 × 80. A low TE 0.6 ms, low-resolution 64 × 48 FLASH image series was also acquired. The AIF was measured from the low-dose high-resolution series (DB), the high-dose low-resolution series (DS), and the high-dose high-resolution conventional single-bolus series (SB). Myocardial time intensity curves were analyzed on a mid-slice based on 6 transmural sectors and quantified by model-constrained deconvolution.


In canine experiments, the Pearson's correlation between microsphere MBF and DB (r = 0.89, figure-a) and DS (r = 0.89, figure-b) estimates were excellent with small bias in Bland-Altman analysis (bias -0.19 and -0.73 ml/g/min). There was an excellent correlation and reasonable bias between DB and DS estimates of MBF in canines (r = 0.97, figure-c) and patients (r = 0.98, figure-d). However, SB overestimated MBF (bias +2.50 ml/g/min, p < 0.001) despite a good correlation with microspheres (r = 0.88). In human studies, SB also overestimated MBF versus either DB or DS estimates (bias +1.47 and +1.38 ml/g/min, p < 0.001).


The MBF estimates by DB and DS are suitable for CMR perfusion quantification. However, SB experiments have large errors in MBF quantification with the doses and parameters studied.

Figure 1

Authors’ Affiliations

National Heart Lung and Blood Institute, National Institutes of Health, Bethesda, MD, USA
Royal Brompton Hospital, London, United Kingdom
Polytechnique Montreal, Montreal, QC, Canada
Catholic University of America, Washington, DC, USA


© Hsu et al. 2016

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The Creative Commons Public Domain Dedication waiver ( applies to the data made available in this article, unless otherwise stated.