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T1 measurements in a rat model of acute myocardial ischemia/reperfusion
© h-Ici et al. 2016
- Published: 27 January 2016
- Myocardial Ischemia
- Left Coronary Artery
- Myocardial Reperfusion
- Myocardial Edema
Myocardial ischemia causes local edema, leading to prolongation of both T1 and T2. In clinical MRI, T2-weighted techniques are commonly used to visualize myocardial edema. The aim was to study the acute T1 changes in-vivo in a novel closed chest animal model of myocardial ischemia/reperfusion using T1 mapping by SALLI in order to test if this parametric approach could provide additional diagnostic information as compared to conventional MRI.
5 groups of rats had an inflatable balloon coronary occluder surgically inserted via thoracotomy. They were allowed to recover for 7 days. MRI was performed to obtain baseline measurement of ventricular function. T1 mapping was performed using the Small-Animal Look-Locker Inversion Recovery (SALLI) technique. Short axis SALLI MR imaging was performed using the same short axis orientation in the mid ventricle distal to the occluder, with SALLI parameters as previously described. Without removing the animals from the scanner, the left coronary artery was occluded for 15, 30 or 60 minutes. 2 groups of animals underwent 3 cycles of 5 minutes of preconditioning before 30 and 60 minutes of ischemia. Myocardial T1 was measured was repeated at 15 minutes intervals during the experiment, throughout ischemia and the 90 minutes of reperfusion. MRI was performed on a whole-body 3.0-T MR unit with a dedicated rat coil.
Myocardial T1 increased in the area-at-risk (AAR) within the first 15 minutes of ischemia. T1 values increased slightly with longer periods of ischemia, but did not change with myocardial reperfusion. Preconditioning led to a more gradual and lesser increase in myocardial T1 in the AAR. Changes in T1 persisted at 3 and 7 days.
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