Fig. 5

Orthogonal views of a representative confocal image volume. The extracellular space is clearly visible with the wheat germ agglutinin (WGA) stain (red), including some t-tubule patterns (arrowhead). Cell ends are also visible, making measurement of myocyte length possible, as shown for example by the arrow. Out-of-plane dimension can also readily be measured in orthogonal views. Scale bar: 50 μm