Fig. 1

Study protocol. Healthy subjects were included in this study to undergo three separate cardiovascular magnetic resonance (CMR) studies. For each participant, all CMR scans were performed on the same day. After the baseline scan in the morning hours, the subjects fasted for 12 h (no food and no water). Within these 12 h the subjects had their normal daily routine. In the evening, a dehydration scan was performed. Afterwards, the participant had access to a sandwich and water, and they were advised to drink at least 1.5 l of water. After drinking this amount, the final hydration scan was performed. On every CMR scan, native T1 and T2 mapping was acquired