Fig. 2

Layer-specific contours in long axis cine images, at endocardial (red), epicardial (green) and equally spaced intramyocardial contours (blue) at end-diastole and end-systole used for the calculation of layer-specific strain. A subset of the 20 total intramyocardial contours used for calculation of average strain are shown. Sample tracings for a four-chamber view are shown for a health control (top) and a patient with heart failure (HF) with preserved ejection fraction (HFpEF) (bottom). Strain values at each layer over the full cardiac cycle are shown on the right