Fig. 5

a Representative long axis view of in vivo (in-plane spatial resolution = 57 µm) and ex vivo (in-plane spatial resolution = 30 µm) high resolution images showing detailed myocardial structure indicating hypertrophy. Right: A schematic to illustrate the relationship of hypertrophy, interstitial fibrosis, perivascular fibrosis and microvasculature. b Representative immunofluorescence staining of mouse heart LV sections for Isolectin B4 and the quantification of the capillary density ratio (number/5 imaging area). Scale bar = 50 μm; Data are shown as the mean ± SD (n = 3 each sex groups; 6 B6 and 6 D2 mice, respectively). *P < 0.05 (t-test) c Sirius red staining show interstitial and perivascular collagen deposition. Upper row images scale bar = 500 µm; lower row images scale bar = 100 µm. Red arrow depicts perivascular fibrosis and blue arrow depicts interstitial fibrosis. Collagen volume fraction (CVF) and perivascular fibrosis ratio (PFR) in septal and inferior area of B6 (n = 8) and D2 (n = 8) mice left ventricle. Data are shown as the mean ± SD. **p < 0.01, using t-test with Mann Whitney test