Skip to main content

1082 Free-breathing single-shot DENSE myocardial strain imaging using deformable registration


Free-breathing scans are often desirable in patients who find breath-holding difficult. We present a new approach for free-breathing myocardial strain imaging with displacement-encoding (DENSE) [1]. It acquires images with a single-shot sequence and removes respiratory motion using deformable registration.


All volunteers (n = 4) were scanned on a 1.5 T Siemens Avanto scanner. A multi-slice, single-shot DENSE sequence (Fig. 1) with true-FISP readout (Fig. 2) [2, 3] and FOV-selective excitation [4] was used to acquire 3 short-axis slices in a heartbeat. Ramped flip-angles through the readout train equalized the echo amplitudes. Displacement-encoding and image acquisition were placed at end-systole and late-diastole respectively, in order to capture the accumulated wall strain of systolic contraction. Imaging parameters were true-FISP echo spacing of 2.5 ms, matrix size of 128 × 40, FOV of 512 × 120 mm with restricted excitation (equivalent to a matrix of 128 × 96 with 3/4 phase-encode FOV), slice thickness of 6–8 mm, and ECG triggering every 2 RR intervals. A data set contained 270 images of 3(slices) × 3(encoding directions) × 30(repetitions). The encoding directions were oblique and combined in-plane encoding moments of 4.0 mm/radian with a through-slice (Z) moment of 1.0 mm/radian. This Z moment was sufficient to suppress the unwanted DC and conjugate-echo signal in the single-shot sequence [4, 5]. Each direction was acquired in a separate heartbeat. The total scan time was 3 – 4 minutes.

Figure 1

Time line of left ventricle contraction, ECG and the sequence.

Figure 2

True-FISP DENSE sequence.

Image registration used a deformable algorithm [6]. The registered images produced 90 strain maps = 3(slices) × 30(repetitions). These were averaged over the repetitions to produce average strain maps for the slices. A correlation threshold was used to remove occasional images of poor registration quality due to ECG mistrigger.

To evaluate strain noise reduction by this approach, we calculated the spatial variance of the pixel-wise strain values in the LV wall for three processing configurations: 1. single repetitions without averaging; 2. the average strain maps; 3. average strain map of respiratory gated (RG) data by selecting 1/3 of the repetitions with the highest diaphragm positions (end expiration).


A typical circumferential strain map from a single repetition and the corresponding average strain map of all repetitions show marked difference in the noise level (Fig. 3). The spatial variance of strain decreased from 0.127 to 0.063 from single repetition to the average of the RG group, and further down to 0.054 upon registration and averaging of all repetitions. The remaining variance may account for real variation around the LV.

Figure 3

Left: Strain map from a single measurement; Right: strain map from the average of all registered images.

Conclusion and discussion

Deformable registration of single-shot DENSE images permits averaging over all of the respiratory cycle for noise reduction. Although the resolution of 4.0 mm is low, the pixel-wise measurement obviates the need for ROI analysis.


  1. 1.

    Aletras AH, et al: Displacement encoding in cardiac functional MRI (abstr). Proc ISMRM. 1998, 1: 281-

    Google Scholar 

  2. 2.

    Bennett E, et al: Ultrafast DENSE technique for mapping the volumetric 3D wall motion of the left ventricle (abstr). Proc ISMRM. 2002, 10: 775-

    Google Scholar 

  3. 3.

    Kim D, et al: Increasing the SNR and data acquisition efficiency of displacement-encoded MRI by the use of TrueFISP imaging at 3 T and TSENSE parallel imaging (abstr). Proc ISMRM. 2006, 3597-

    Google Scholar 

  4. 4.

    Aletras AH, et al: High-resolution strain analysis of the human heart with fast-DENSE. JMR. 1999, 140 (1): 41-57. 10.1006/jmre.1999.1821.

    CAS  Article  PubMed  Google Scholar 

  5. 5.

    Zhong XD, et al: Selective suppression of artifact-generating echoes in cine DENSE using through-plane dephasing. MRM. 2006, 56 (5): 1126-1131.

    Article  PubMed  Google Scholar 

  6. 6.

    Hermosillo G, et al: Variational methods for multimodal image matching. IJCV. 2002, 50 (3): 329-343. 10.1023/A:1020830525823.

    Article  Google Scholar 

Download references

Author information



Corresponding author

Correspondence to Yuan Le.

Rights and permissions

Open Access This article is published under license to BioMed Central Ltd. This is an Open Access article is distributed under the terms of the Creative Commons Attribution 2.0 International License (, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Reprints and Permissions

About this article

Cite this article

Le, Y., Kellman, P., Bennett, E.E. et al. 1082 Free-breathing single-shot DENSE myocardial strain imaging using deformable registration. J Cardiovasc Magn Reson 10, A207 (2008).

Download citation


  • Noise Reduction
  • Circumferential Strain
  • Processing Configuration
  • Respiratory Gated
  • Single Repetition