2101 Role of hemodynamic forces on plaque development: non-invasive assessment with MRI methods
© Zheng et al; licensee BioMed Central Ltd. 2008
Published: 22 October 2008
Fluid shear stress was thought to lead to atherosclerotic plaque progression, but such measurement procedures are often invasive. The goal of the present study is to address the biomechanical pathways of atherosclerosis progression and regression with our non-invasive MRI methods and a 3D fluid-structure interaction (FSI) model. The study was performed in a large animal atherosclerotic model.
Five pigs were fed cholesterol-enriched diets (HC) and then underwent balloon overstretch injury to the left carotid artery to simulate angioplasty or stent restenosis. A plaque-targeting contrast agent, Gadofluorine (Bayer Schering Pharma AG, Germany) enhanced MRI scans were performed at various time points after the balloon injury while the pigs were maintained on HC. The atherosclerotic-like carotid lesions in four of the pigs were then re-injured by balloon angioplasty. MRI scans were repeated to monitor the progression and regression (by feeding normal chow diet to the pigs) of the plaques. The pigs were euthanized after 5 to 9 months and the left and right (normal) carotid arteries were dissected for histopathology and mechanical testing to obtain subject-specific material properties.
In each MRI study, Gadofluorine was injected intravenously at a dose of 0.05 – 0.075 mmol/kg one hour before each MRI scan. The MRI (at 3-T, Siemens Trio MRI System) protocol consisted of 20-slice T1w and T2w imaging using dark-blood turbo-spin-echo (DB-TSE) sequence, as well as a 20-slice 3D gradient-echo (GRE) imaging. The slice thickness was 2 mm and the true image resolution was 0.33 mm × 0.33 mm. The left carotid vessel wall and plaque images were segmented based on contrast enhancement patterns. Using the material parameters obtained from mechanical testing, the 3D FSI computational model that was validated in an in vitro study was applied to data from two pigs to calculate various structure stress and strain distribution, including maximal fluid shear stress, within the plaques at different time points.
Gadofluorine enhanced MRI and 3D computational models may allow non-invasive dynamic assessment of biomechanical behaviors of atherosclerotic plaques in vivo. Structure stress and strain may play an important stimulation role for plaque regression. This more comprehensive information would provide useful insight to the plaque development and help select appropriate interventions to avoid subsequent cardiac events.
This article is published under license to BioMed Central Ltd.