- Meeting abstract
- Open Access
152 Gadolinium-labeled liposomes as a DENSE-compatible, positive MRI contrast agent for serially assessing macrophage activity and regional contractile function in the murine heart after myocardial infarction
© French et al; licensee BioMed Central Ltd. 2008
- Published: 22 October 2008
- Myocardial Strain
- Liposome Dispersion
- Multilamellar Liposome
- Regional Contractile Function
- Peak Circumferential Strain
Monocyte/macrophage activity in the left ventricle (LV) post-myocardial infarction (MI) has previously been assessed using small/ultra-small paramagnetic iron oxide (SPIO/USPIO) particles to yield negative contrast on CMR images. However, the susceptibility-induced signal loss from iron interferes with the quantitation of myocardial strain in the very regions of interest occupied by the iron particles.
To develop a new multimodal, multilamellar liposomal agent labeled with both Gd-DTPA (for CMR imaging) and a fluorescent tag (DiI-DS) that provides for serial macrophage tracking without interfering with cine DENSE analysis of contractile function.
Multimodal, multilamellar liposomes (MML) were prepared from 1-palmitoyl-2-oleoyl-3-sn-glycerophosphocholine and cholesterol (1:1 molar ratio), with the addition of N-methoxy-poly(ethyleneglycol)-1,2-distearoyl-3-sn-phosphoethanolamine (<1 mol%), Gd-DTPA-bis-stearylamide (7 mol%, Sigma Chemical) and DiI-DS (0.2 mol%, Molecular Probes). The liposome dispersion was then passaged multiple times through 0.4 um filters to obtain a mean diameter of ~0.3 um as determined by dynamic light scattering. Nine C57Bl/6 mice were subjected to 1 h of coronary occlusion and up to 7 d of reperfusion. MML volumes of 50 μl were injected IV on Day 2 post-MI for subsequent phagocytosis by monocytes/macrophages. Mice were studied by CMR 2–4 h after Day 2 injection and thereafter on a daily basis until Day 7 post-MI. CMR included inversion-recovery (IR) to image MML followed by IP infusion of Gd-DTPA and more IR to image the entire infarcted zone. In a subset of these studies, cine DENSE MRI  was performed on mid-ventricular short-axis slices prior to the IP infusion of Gd-DTPA. CMR was performed on 4.7 T Varian and 7 T Bruker/Siemens ClinScan MRI systems. Cine DENSE was performed on the ClinScan. Blood was drawn at regular intervals between 15 min and 6 h post-injection to assess DiI fluorescence by FACS and microplate reader. After the last imaging session, hearts were removed for Mac2 immunostaining, microscopy and FACS.
Multimodal, Multilamellar Liposomes are useful for assessing monocytes/macrophage infiltration into the infarcted LV where they remain detectable until at least Day 7 post-MI. The integral Gd-DTPA tag provides for highly-specific positive MR contrast while the fluorescent tag facilitates FACS analysis and histological confirmation via fluorescence microscopy. The MML contrast agent represents a significant advance over conventional iron-based agents, because the absence of iron enables the complementary use of myocardial strain imaging by techniques such as cine DENSE. Employed together, these methods can be used to non-invasively assess the impact of inflammatory cells on regional contractile function in vivo.