- Oral presentation
Non-invasive monitoring allograft rejection by simultaneous cellular and functional cardiac MRI
Journal of Cardiovascular Magnetic Resonancevolume 11, Article number: O79 (2009)
The current gold standard for diagnosing and staging rejection after organ transplantation is biopsy, which is not only invasive, but is also prone to sampling errors. The regimen for treating acute rejection after heart transplantation varies among treatment centers, partly due to lack of sensitive and reliable indices for assessing the status of myocardial rejection. The goal of this study is to establish sensitive and reliable indices using cellular and functional MRI for non-invasive detection of acute cardiac allograft rejection after heart transplantation. Using a rodent model of cardiac transplantation, we monitor both immune cell infiltration and cardiac dysfunction resulting from rejection in the same imaging session. Immune cells, mainly monocytes and macrophages, are labeled in situ with dextran-coated ultra-small superparamagnetic iron oxide (USPIO) nano-particles. T2*-weighted MRI and strain analysis of tagged MRI are used to correlate immune-cell infiltration and ventricular function with rejection grades.
1. Animal model
An abdominal heterotopic working heart and lung transplantation model with DA to BN transplantation rat pairs was used. The transplanted hearts exhibit cardiac outputs and ventricular pressure similar to those of native hearts. In this model, mild (Grade 1A or B) rejection develops by post-operation day (POD) 2.5–3.5, Grade 2 rejection develops on POD 4.5–5.5, and moderate to the severe (Grade 3A) rejection develops after POD 6–7.
2. MRI methods
ECG and respiration gated T2*-weighted cine imaging on Bruker AVANCE 4.7-T system was used for in-vivo imaging with an in-plane resolution of 156 μm. Tagged MRI is achieved with a modified DANTE sequence. Strains were analyzed by the HARP method with software obtained from Diagnosoft, Inc.
3. Iron-oxide particle labeling
Immune cells, mostly macrophages, are labeled in situ by direct intravenous injection of USPIO particles 1 day prior to MRI scans.
Immune cells, mainly monocytes and macrophages, take-up circulating USPIO particles by endocytosis. Foci of labeled immune cells in the rejecting graft are observed by regions of hypointensity in T2*-weighted images (Fig. 1). The observed immune-cell infiltration is heterogeneous, thus it is not surprising to have incidences of false-negative results with biopsy. Monitoring immune-cell infiltration with MRI is not only non-invasive, but also provides whole-volume 3D perspective of rejection.
To discern if the heterogeneity of rejection is manifested in cardiac function, tagged MRI is used to monitor regional ventricular contractile function. Although global function may appear normal, the rejected allograft may show local hypokinesis (Fig. 2D). Strain analysis by HARP was used to quantify the local contractile function. Regions with compromised strains (Fig. 2E &2F) largely correlate with the areas of higher macrophage infiltration.
For easier visualization of the regions with compromised strains, 48 probe-points were placed evenly throughout LV, starting at the anterior intercept of LV and RV (Fig. 2F & Fig. 3A). The probe-point is considered "compromised" if the strain value of the particular location deviats from the mean isograft values by more than 1 standard deviation; otherwise the probe-point is scored as "normal" (Fig. 3B). The number of compromised probe-points is well correlated with rejection grades (Fig. 3C), which can potentially be useful clinical index for rejection.
In our model, acute allograft rejection after heart transplantation is spatially heterogeneous, which is manifested in both immune-cell infiltration and ventricular function. Cardiac MRI is both non-invasive and provides 3D, whole-heart perspective of rejection status, which potentially allows more reliable detection of acute allograft rejection.