- Poster presentation
- Open Access
Murine Es-derived cardiomyocytes form grafts and improve cardiac function in the infarcted myocardium
© Zhang et al; licensee BioMed Central Ltd. 2009
- Published: 28 January 2009
- Embryonic Stem Cell
- Embryonic Stem Cell Line
- Murine Embryonic Stem Cell
- Vehicle Treated Animal
- Global Cardiac Function
embryonic stem cells (ESC) readily differentiate into cardiac lineage making them a potential source of transportable cells for myocardial regeneration. However the low yield of ESC-derived cardiomyocytes (ESC-CMs) using the conventional differentiation method makes it difficult to perform in vivo study and low enrichment of CMs leads to concerns of teratoma formation.
a murine ESC line containing puromycin resistance gene under control of a cardiac specific promoter, sodium calcium exchanger (NCX) was used to generate ESC-CMs. ESC-CMs were labeled with Superparamagnetic iron-oxide nanoparticles (SPIO) for MRI detection. Reperfused myocardial infarction was induced in athymic rats. Infarction size was estimated by MRI post-op day1 to exclude animals with infarct size smaller than 10% or larger than 35%of the LV volume. At post-op day 7, labeled ESC-CMs (5–10 millions) were injected into infarction region. Control group was injected with vehicle. MRI scan was performed at post-op day 8 to confirm successful CM cell transplantation. Global cardiac function in ESC-CM and vehicle treated animals was assessed by MRI for 2 months. Immunohistology staining and electrophysiology were performed on postmortem hearts and ESC-CMs, respectively.
Large numbers of highly pure ESC-CMs were obtained. Preliminary results suggest that ESC-CMs form grafts and improve LV function in the infarcted hearts.
This article is published under license to BioMed Central Ltd.