- Poster presentation
- Open Access
MR imaging of human atherosclerosis using immunomicelles molecularly targeted to macrophages
© Amirbekian et al; licensee BioMed Central Ltd. 2009
- Published: 28 January 2009
- Inductively Couple Plasma Mass Spectroscopy
- Lipid Core
- Severe Atherosclerosis
- Confocal Fluorescent Microscopy
- Clinical Scanner
Early assessment of atherosclerosis (leading cause of death in West and soon world) remains an elusive clinical goal, which if realized could lead to significant improvements in mortality and morbidity.
Gadolinium (Gd)-containing immunomicelles targeting macrophages improved magnetic resonance (MR) detection of murine atherosclerosis. We sought to determine if immunomicelles targeting the macrophage scavenger receptor-B (CD36) improved ex-vivo MR detection and characterization of human aortic atherosclerosis.
Gd-containing micelles, anti-CD36 immunomicelles and Fc-micelles were created. Macrophages were incubated with fluorescent micelles and immunomielles to determine uptake via confocal microscopy and inductively coupled plasma mass spectroscopy (ICP-MS) was performed to quantify Gd uptake. Human aortic specimens with moderate to severe atherosclerosis were harvested at autopsy. Using a 1.5 T Siemens clinical scanner, T1, T2, and PDW 3-dimensional scans were performed and post-contrast scans were repeated after 24 h incubation. T1 analysis and cluster analysis were performed comparing immunohistopathology with MR images. P-values < 0.05 were considered significant.
Macrophage-specific (CD36) immunomicelles bind to human macropages in vitro and improved MR detection and characterization of human aortic atherosclerosis. Thus, immunomicelles could help identify high-risk human plaque.
This article is published under license to BioMed Central Ltd.