- Technologist presentation
- Open Access
Dual PARACEST and 19F MR molecular imaging of fibrin clots with targeted perfluorocarbon nanoparticles
© Cai et al; licensee BioMed Central Ltd. 2009
- Published: 28 January 2009
- Nanoparticle Concentration
- Fibrin Clot
- Chemical Exchange Saturation Transfer
- Total Imaging Time
- Increase Nanoparticle Concentration
Fibrin is an abundant component of thrombi and an early marker of ruptured atherosclerotic plaques, which are the major cause of myocardial ischemia and stroke. Identification of fibrin could help detect ruptured plaques and direct therapeutic interventions to prevent or ameliorate the consequences of a heart attack or stroke. Fibrin-targeted perfluorocarbon nanoparticles provide a unique platform for molecular imaging of thrombi by MRI. The particle surface can be formulated with MRI contrast agents, such as PARACEST (PARAmagnetic Chemical Exchange Saturation Transfer) chelates that can provide "contrast on demand" by simply turning on and off a prepulse. In addition, the perfluorocarbon core can be exploited for 19F imaging.19F offers high signal intensity and no background signal from biological tissues, yielding a unique signature.
To combine PARACEST and 19F imaging for the corroborative detection of thrombi and quantitating the binding of targeted nanoparticles.
Dual PARACEST and 19F MR molecular imaging of fibrin with targeted PARACEST perfluorocarbon nanoparticles was demonstrated at 11.7 T. Phantoms showed the detection limit of the PARACEST nanoparticles was <500 pM. The PARACEST enhancement observed on the clot surface was higher than the expected value based on the 19F signal, suggesting that binding the nanoparticles to a target improves the efficacy of the contrast mechanism.
This article is published under license to BioMed Central Ltd.