- Moderated poster presentation
- Open Access
Whole-heart T2-weighted (T2w) sequence for imaging post-infarct edematous area at risk (AR) in mice
© Beyers et al; licensee BioMed Central Ltd. 2010
Published: 21 January 2010
T2w cardiac magnetic resonance (CMR) defines the area at risk (AR) in large mammals by imaging post myocardial infarct (MI) edema. Here, we expand the T2w CMR to cover the entire left ventricle (LV) in mice and jointly applied late gadolinium enhanced (LGE) CMR to non-invasively define MI size as percent AR. Fast murine heart rate, flow and motion present challenges to T2w CMR success. The typical 60 ms echo time for edema contrast occupies a large portion of the murine cardiac cycle which prohibits traditional T2w methods.
Develop a whole-heart, multi-slice, T2w CMR sequence for mice with high immunity to flow and tissue motion artifacts, has dark-blood suppression and maintains sufficient signal-to-noise (SNR) and contrast-to-noise (CNR) performance.
Our non-selective T2prep sequence employed a Malcolm Levitt-weighted (MLEV-14) composite C180x (90x:180y:90x) refocus pulse train followed by a multi-slice, gradient-echo readout sequence. Flow-sensitization gradients were placed optimally during the T2prep to provide dark-blood capability. We imaged three mice on Days 1 thru 4 (D1-4) after reperfused MI induced by 60-minute coronary occlusion. Parameters included TR = 3 sec, TE = 60 ms, FOV = 25 × 25 mm, slice thickness = 1 mm, matrix = 128 × 128, BW = 520 Hz/pixel. LGE CMR was also performed on D1 to define MI size after T2w CMR was completed. Scans were performed on a Bruker 7 T ClinScan that covered the entire LV in 6 or 7 contiguous short axis slices.
T2w CMR in mice yields results consistent with those in larger mammals. T2w CMR can now be applied in knock-out mice to study the influence of individual genes and treatments on MI size measured as percent area at risk.
This article is published under license to BioMed Central Ltd.