- Oral presentation
- Open Access
MRI and CT tracking of mesenchymal stem cells with novel perfluorinated alginate microcapsules
© Fu et al; licensee BioMed Central Ltd. 2010
- Published: 21 January 2010
- Mesenchymal Stem Cell
- Medial Thigh
- Imaging Contrast Agent
- Alginate Microcapsule
- Cell Microencapsulation
Stem cell therapies, although promising for treating ischemic arterial diseases, suffer from poor engraftment and the inability to noninvasively monitor and track transplanted cells in vivo. Stem cell microencapsulation in conjunction with an imaging contrast agent provides a means to prevent cell immunorejection and enable cell tracking with appropriate imaging modalities. The objective of this study was to design and evaluate a novel MRI- and CT-visible, immunoprotectable alginate microcapsule containing an imaging contrast agent, perfluorooctylbromide (PFOB), for mesenchymal stem cell (MSC) delivery.
Microencapsulation of bone marrow-derived rabbit MSCz (1.5 × 106cells/ml) was performed using a modified cell microencapsulation method with the addition of PFOB. MSCs viability was determined using a fluorometric assay. In vitro phantom studies using 19F MRI and c-arm CT imaging were performed to determine the minimum detectable number of PFOB Caps using standard clinical imaging systems. New Zealand White (NZW) rabbits (n = 16) were randomized to receive 6 injections (~500 capsules/injection) of unlabeled microcapsules, PFOB Caps, or naked MSCs in the medial thigh. X-ray angiograms, c-arm CT, and 19F MR images were taken within 1-14 days after injection in a rabbit PAD model. Intensity-weighted centroids of each injection site from CT images and postmortem 3D rendering volumes were determined after coregistration using 3D registration software (Dextroscope). Registration error was quantified by linear distance between paired CT and postmortem injection sites.
PFOB microcapsules provide an ideal microenvironment for maintaining MSC viability in vitro, while enabling the monitoring of MSC delivery and tracking of engraftment in vivo using clinical MRI and CT imaging systems.
This article is published under license to BioMed Central Ltd.