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- Open Access
Quantitative molecular imaging of atherosclerotic endothelial dysfunction with perfluorocarbon (19F) nanoparticle magnetic resonance imaging and spectroscopy
© Zhang et al; licensee BioMed Central Ltd. 2010
Published: 21 January 2010
Disturbed endothelial barrier function in atherosclerosis has been detected by MRI by imaging gadolinium leakage into the vascular interstitium but not yet quantified.
We propose that the unique, no background 19F signal from crown ether perfluorocarbon-core nanoparticles (NP: ~250 nm) might both visualize and quantify endothelial disruption in advanced atherosclerosis.
Five NZW rabbits were fed a high fat diet for 9-12 months (cholesterol: 1200-1700 mg/dL). Fluorescently-labeled, nontargeted NP were injected (2 ml/kg) intravenously into rabbit ear vein. After circulation in vivo for 1, 6 or 24 hours, aortas were excised for 19F MRI and spectroscopy (Varian 11.7 T scanner); and whole mount fluorescence imaging (Xenogen IVIS system). Two human carotid endarterectomy tissues were collected from operation room. After pretreatment with plasmin to digest fibrin on the endothelial surface and incubation with nontargeted NP for 6 hours, tissues were rinsed and formalin fixed for 19F MRI and spectroscopy. A perfluorooctyl bromide standard enabled MRS-based quantification of NP concentration in each imaged voxel.
For both advanced experimental animal atherosclerosis and native human atherosclerosis tissues, nontargeted NP rapidly penetrate the leaky endothelial barrier, which can be visualized and quantified ex vivo with the use of "no background" 19F MRI and MRS. This experimental strategy offers a potential new approach for quantification of endothelial dysfunction employing both in vivo and ex vivo incubation with nanoparticle tracers.
This article is published under license to BioMed Central Ltd.