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Reproducibility of First Pass Perfusion CMR at rest and during hyperaemia for estimation of myocardial perfusion


CMR has demonstrated excellent reproducibility for assessment of cardiac function, mass and volume. Reproducibility data for first-pass CMR perfusion estimates are sparse, but are important to monitor for example the response of therapies which affect myocardial blood flow.


1. To assess intraobserver, interobserver and interstudy reproducibility of myocardial blood flow estimation from first-pass perfusion CMR in at rest and during hyperaemia.

2. To compare semi-quantitative and quantitative measurements.


11 volunteers (6 males, mean age 33 ± 7 years) underwent CMR perfusion on 2 separate days (mean interstudy delay = 84 ± 111 days, median delay = 7 days) on a 1.5 T Philips Intera system during adenosine-induced hyperaemia (140 mcg/kg/min, 0.05 mmol/kg Gd-DTPA) and rest. A pulse sequence optimised for acquisition of a single midventricular slice at systole was used (saturation recovery segmented gradient echo, 2 × SENSE TR/TE/flip 2.7 ms/1.0/15°, typical FOV 380 × 380 mm, matrix 160 × 160, slice thickness 10 mm, preparation pulse delay 150 ms, shot duration 130 ms). Endo- and epicardial contours were drawn. "Semi"-quantitative analysis calculated maximal upslopes of myocardial signal intensity of the entire slice, normalised to the LV blood-pool (Myocardial perfusion index, MPI) using MASS 6.0 (Medis, Leiden, The Netherlands). The myocardial perfusion reserve index (MPRI) was calculated as the ratio of hyperaemic over rest MPI.

Data generated in MASS were imported to a Fermi Function deconvolution algorithm implemented in Matlab (The MathWorksInc, USA) [1], that provided estimates of absolute myocardial blood flow (ml/g/min) with user input to correct for baseline and timing offsets between the input and tissue response functions. All measurements were performed by one observer, who repeated analysis of the first CMR scan after 4 weeks. In addition, a second observer performed separate blinded analysis of the first study.


All measurements indicated good reproducibility of global CMR perfusion estimates (Table 1). Reproducibility was highest for intraobserver followed by interobserver cmparisons especially in stress studies and was lowest for interstudy comparisons. Semiquantitative measurements were generally more reproducible than fully quantitative analysis.

Table 1 Intraobserver, Interobserver and Inter-study reproducibility of CMR


1. First-pass perfusion CMR with "semi"-quantitative and quantitative analysis has comparable reproducibility to other imaging modalities [2].

2. The lower interstudy reproducibility probably reflects at least in part physiological heterogeneity.

3. The higher variability of quantitative analysis in this study was probably the result of the cumulative variability of segmentation and subsequent Fermi-deconvolution with user input at both stages.


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Open Access This article is published under license to BioMed Central Ltd. This is an Open Access article is distributed under the terms of the Creative Commons Attribution 2.0 International License (, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Larghat, A.M., Radjenovic, A., Maredia, N. et al. Reproducibility of First Pass Perfusion CMR at rest and during hyperaemia for estimation of myocardial perfusion. J Cardiovasc Magn Reson 12 (Suppl 1), P228 (2010).

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