Cardiac metabolism with hyperpolarized [1-13c]pyruvate: a feasibility study in mini-pig with a large dose injection
- Luca Menichetti1,
- Francesca Frijia2,
- Vincenzo Lionetti3,
- Maria Filomena Santarelli1,
- Jan Henrik Ardenkjaer Larsen4,
- Giulio Giovannetti1,
- Vincenzo Positano2,
- Daniele De Marchi2,
- Giovanni Aquaro2,
- Manuela Campan3,
- Valentina Hartwig5,
- Matteo Milanesi2,
- Fabio A Recchia6,
- Luigi Landini2 and
- Massimo Lombardi2
© Positano et al; licensee BioMed Central Ltd. 2010
Published: 21 January 2010
MRI with hyperpolarised 13C represents a promising modality for in vivo spectroscopy and it could provide a unique opportunity for non invasive assessment of cardiac regional metabolism.
The aim of this work is to study real time in vivo cardiac metabolism after intravenous (i.v.) injection of hyperpolarized [113C]-pyruvate in a large animal model with a clinical 3T scanner.
Four normal male mini-pigs (35 kg) were maintained under deep sedation with midazolam (0.1 mg/kg/h i.v.) while ECG, temperature and arterial blood pressure were monitored (SA Instruments, New York USA ).
13C-1-pyruvate Hyperpolarization was performed using Dynamic Nuclear Polarization (Hypersense, Oxford Instruments, Oxford, UK). The final injection solution contained 230 mM sodium [1-13C]pyruvate, 100 mM TRIS buffer, 0.27 mM Na2EDTA and 20 microM Dotarem (Guerbet, Paris, France). Temperature of solution was about 37ºC and pH = 7.6. A dose of 20 mL was administered over 10 s by manual injection.
The mini-pigs underwent both 1H MR imaging and hyperpolarized 13C MRS. The experiments were performed with a 3 T GE Signa HDx (GE Healthcare, Waukesha, WI, USA) scanner with a 13C quadrature birdcage coil (Rapid Biomedical, Würzburg, Germany). Anatomical imaging was acquired with the body coil and FIESTA sequence (FOV=35, FA=45, TE/TR=1.71ms/3.849ms).
13C dynamic spectra were acquired using elliptic-FIDCSI pulse sequence (bandwidth 5000Hz, 2048 pts, 10º FA). A long-axis slice of 20 mm was selected during excitation. Spectra covering the heart were acquired from the beginning of the injection of the hyperpolarized [1-13C]pyruvate, every 2 s, for 120 s.
Data processing was performed by using MATLAB (The Mathworks, Inc., Natick, MA) and jMRUI software tools. The dynamic spectra were phase corrected by adjusting both zero-and first-order frequency-dependent phase components. Pyruvate, pyruvate hydrate, alanine, lactate, and bicarbonate were estimated by using AMARES algorithm included into jMRUI tools. Accordingly, time courses of metabolites are generated.
Results and discussion
Imaging cardiac metabolism with hyperpolarized 13C is feasible with 3T MRI in an animal model that closely resembles the human heart phenotype.
This article is published under license to BioMed Central Ltd.