- Open Access
Non-triggered quantification of central and peripheral pulse-wave velocity
© Langham et al; licensee BioMed Central Ltd. 2011
- Received: 11 August 2011
- Accepted: 21 December 2011
- Published: 21 December 2011
Stiffening of the arteries results in increased pulse-wave velocity (PWV), the propagation velocity of the blood. Elevated aortic PWV has been shown to correlate with aging and atherosclerotic alterations. We extended a previous non-triggered projection-based cardiovascular MR method and demonstrate its feasibility by mapping the PWV of the aortic arch, thoraco-abdominal aorta and iliofemoral arteries in a cohort of healthy adults.
Materials and Methods
The proposed method "simultaneously" excites and collects a series of velocity-encoded projections at two arterial segments to estimate the wave-front velocity, which inherently probes the high-frequency component of the dynamic vessel wall modulus in response to oscillatory pressure waves. The regional PWVs were quantified in a small pilot study in healthy subjects (N = 10, age range 23 to 68 yrs) at 3T.
The projection-based method successfully time-resolved regional PWVs for 8-10 cardiac cycles without gating and demonstrated the feasibility of monitoring beat-to-beat changes in PWV resulting from heart rate irregularities. For dul-slice excitation the aliasing was negligible and did not interfere with PWV quantification. The aortic arch and thoracoabdominal aorta PWV were positively correlated with age (p < 0.05), consistent with previous reports. On the other hand, the PWV of the iliofemoral arteries showed decreasing trend with age, which has been associated with the weakening of muscular arteries, a natural aging process.
The PWV map of the arterial tree from ascending aorta to femoral arteries may provide additional insight into pathophysiology of vascular aging and atherosclerosis.
- Cardiovascular Magnetic Resonance
- Aortic Arch
- Arterial Segment
- Pulse Transit Time
- Muscular Artery
In elastic arteries, e.g. aorta and carotid arteries, the repeated mechanical loading fragments and degrades elastin and is replaced by much stiffer collagen giving rise to decreased wall distensibility . Aortic stiffness can be assessed via quantification of pulse-wave velocity (PWV), which is defined as the rate at which blood motion is transmitted. It is typically quantified by measuring the time delay of the systolic pressure wave at some downstream location, using pressure transducers [2, 3] or Doppler US  placed at the two locations (typically carotid and common femoral arteries). The method has significant limitations in that the actual path length of the wave is not known. Further, arterial tortuosity increases with age and can vary from subject to subject . By contrast, cardiovascular magnetic resonance (CMR) allows an accurate measurement of the path length and provides regional differences in aortic stiffness [6–9]. Cardiac-gated phase contrast CMR in the sagittal plane [8, 10], "pencil beam" excitation [11, 12] or sinusoidal tags of a column of blood  has been be used to evaluate the thoraco-abdominal aorta but implementing these methods for the peripheral arteries may not be feasible due to increased tortuosity and smaller diameter. On the other hand, cardiac-gated phase contrast CMR in the axial plane  can be extended to the peripheral arteries since the pressure pulse transit time will increase distally with respect to the cardiac trigger.
Unlike a central artery, there is sparse PWV data on viscoelastic peripheral arteries (e.g. brachial and femoral arteries) that have higher smooth muscle content. In contrast to the aorta, data suggest (based on ultrasound methods) that the distensibility of muscular arteries remains constant with age [15–19]. According to the Moens-Korteweg formula, , the age-related effect on the peripheral PWV will be absent, where D is the distensibility coefficient  and ρ is the density of blood. Quantification of distensibility requires measurement of the change in the artery's diameter between systole and diastole and is related to the frequency independent component of the vessel wall's dynamic modulus [6, 20]. On the other hand, the wave-front velocity approach, commonly known as the "foot-to-foot" method, inherently captures the high-frequency component of the dynamic modulus associated with the rapid increase in pressure during early systole. The dynamic modulus of elastic and viscoelastic arteries has different response to oscillatory pressure waves created by pulsatility. For elastic arteries (e.g. carotids and aorta) PWV quantification by both methods is expected to give approximately the same result since there is little or no lag between the changes in lumen diameter in response to a pressure increase. Muscular arteries such as femoral and brachial arteries have much higher smooth muscle content, which confers their viscoelastic properties. The dynamic modulus at 10 Hz can be 3 to 4 times greater than at 1 Hz in young subjects (<20 yrs), whereas the modulus plateaus at 1 Hz in older (> 35 yrs-old) subjects . Thus, the estimation of stiffness or PWV based on distensibility estimation may not be optimum for assessing structural remodeling of muscular arteries associated with aging or endothelial dysfunction.
In this work we extend the previously developed projection-based approach  to estimate PWV along the thoraco-abdominal aorta and the iliofemoral arteries by "simultaneously" exciting and collecting velocity-encoded projections at two arterial segments. The method achieves high temporal resolution without gating by sampling the center k-space line repeatedly to approximate the velocity-time curve during the cardiac cycle from a measurement of the complex difference (CD) signal intensity. The goal of this work is to demonstrate the feasibility of the proposed method in healthy subjects.
Non-triggered quantification of PWV with velocity-encoded projections
The CD between velocity-encoded projections retains signal from the moving spins only and represents average signal across the lumen in the projection direction (perpendicular to the readout direction). In order to avoid interference from nearby vessels a suitable readout direction must be chosen. At the onset of systole (flow velocity << v max ) CD intensity is approximately proportional to velocity, thus the wave-front velocity method can be applied to the time-resolved CD curves to estimate the wave-front propagation time. For example, sin(φ/2) and φ/2 will differ by only 6% even at v = 0.5v max for an encoding velocity (VENC) ~1.33v max .
Written informed consent was obtained prior to all examinations following an institutional review board-approved protocol. Ten healthy subjects (N = 10, age range 23 - 68 yrs, mean age 44 ± 14 yrs) without prior history of cardiovascular disease were recruited to evaluate the feasibility of quantifying PWV from aortic arch to iliofemoral arteries.
All studies were performed at 3T system (Siemens Tim Trio) using two body matrix and spine coils. The body matrix coil is a 6-element design with 2 clusters of 3 elements, designed so that each cluster can be manually turned on or off. The elements within each cluster are arranged laterally across the chest or abdomen so that each cluster is sensitive to different region along the head-foot direction. An oblique sagittal image through the aorta is first acquired to prescribe and generate multiple axial slices below the pulmonary trunk. Once a suitable axial slice is selected an appropriate readout direction is chosen to avoid vessel interference in velocity-encoded projections, as described in . The imaging parameters for the quantification of PWV along the aortic arch are similar to the previous studies: FOV = 448 mm, voxel size = 2 ×10 mm2, TE/TR = 2.6/5.0 ms, bandwidth = 893 Hz/pixel, flip angle = 15° and VENC = 175 cm/s (corresponding to 350 cm/s for complex difference). After collecting a reference image (for the purpose of identifying vessels in CD images) 1024 pairs of velocity-encoded projections are acquired in free-breathing mode, covering about 10-12 heart-beats at a temporal resolution of 10 ms. The data acquisition time is under 12 s.
Body matrix coils are placed on the chest and abdomen such that the proximal descending (Da) and abdominal aorta (Aba) proximal to the iliac bifurcation are approximately centered about a cluster of each body coil. Axial scout images are acquired over the maximum FOV allowed (400 mm) with 20 mm gap to prescribe the two desired slice locations typically separated by 200 to 300 mm. Lastly, the midpoint (halfway between the two prescribed slices) is repositioned to the isocenter of the scanner. The imaging parameters are as follows: FOV = 352 mm, voxel size = 1.38 × 10 mm2, TE/TR = 4.0/6.0 ms, bandwidth = 781 Hz/pixel, flip angle = 15o and VENC = 125 cm/s. The increased TR (relative to the one used for the aortic arch) caused by lower VENC results in a temporal resolution of 12 ms but compensated more than enough from the increase in the path length from about 120 mm to about 280 mm for thoracoabdominal aorta. Even though the temporal resolution is reduced by 20% the path length is more than doubled on average thus leading to higher precision compared to measurements at the aortic arch.
The path length L of the wave-front is estimated from the oblique sagittal (aortic arch) and axial scout images (thoraco-abdominal and iliofemoral arteries) acquired previously for prescribing slices of interest. On the sagittal image a center-line of the aorta between the two aortic sites is manually drawn to estimate the path length. In the axial images, the coordinates of the artery's centroid at each slice is recorded to compute the in-plane displacements (Δx, Δy) of the vessel's centroid, where adjacent slices are separated by cm. Thus the path length is given by , where N is the number of axial slices. The PWV is then calculated as, where is the propagation time averaged over multiple heart beats.
This work demonstrates the feasibility of quantifying three regional (aortic arch, thoraco-abdominal aorta and iliofemoral arteries) PWVs by collecting velocity-encoded projections repeatedly at arterial segments of interest. The proposed non-triggered method is less sensitive to patient motion and irregular heart rhythm as PWV is estimated in "real-time" for each heartbeat. Further, the results suggest that the method may be able to capture the age-related increase in the stiffness of the aortic arch and to distinguish differences in the aging process of elastic and muscular arteries. Epidemiological and autopsy studies previously demonstrated a close link between aging and symptoms of atherosclerosis . Thus, the ability to quantify the effect of age on peripheral PWV in conjunction with the behavior of a central artery may provide additional insight into systemic disease. To the best of our knowledge, peripheral PWV has not been quantified using CMR and the transducer approach has primarily targeted the superficial brachial artery. The present study was designed to demonstrate feasibility and was not powered to evaluate the dependence of age on the two different types of arteries.
We observed variation in PWV from one cardiac cycle to another as indicated by the average standard error of about 0.3 m/s (c.f. Figure 7). A few studies have reported heart rate dependence of PWV [27–29] but, to the best of our knowledge, studies involving variations in PWV between cardiac cycles have not previously been reported. The variation in R-R interval at rest is not uncommon and can lead to a different relaxation state of the artery, thus variations in PWV between successive cardiac cycles are expected. Unfortunately, intersubject standard deviations instead of individual standard error are typically reported in the literature even though PWV estimated via tonometry, CMR or Doppler is derived from many cardiac cycles. The heart rate variation cannot fully explain the differences in the PWV between different cardiac cycles. However, we observed that the standard error is smaller in the thoracoabdominal aorta and iliofemoral artery compared to the aortic arch. This is not surprising since the separation distance between the proximal and distal arterial segments are much larger (250 to 350 mm in the thoracoabdominal aorta and iliofemoral artery segments compared to about 120 mm in aortic arch), resulting smaller error in transit time estimation. However, the overall error is reduced by averaging the PWV over 8-10 cardiac cycles as demonstrated previously  in which the projection method was compared directly against the established cine PC-CMR.
The method is limited to slices where vessel interference can be avoided with an appropriate choice of readout orientation. This limitation is exacerbated when projections have to be acquired simultaneously at two slices thus requiring careful planning for prescribing the slices of interest. In the peripheral arteries, vessel overlap with the veins could be ignored considering that the venous CD signal was at the level of noise (c.f. Figures 4a and 4b). We implemented two successive RF pulses instead of modulating the sinc pulse by a cosine function to reduce average and peak SAR. However, the two RF pulses were separated by less than one millisecond and thus the decrease in the CD signal due to relaxation and blood flow motion is negligible.
For elastic arteries such as the aorta, PWV can be quantified with either the foot-to-foot approach or by estimating distensibility. In fact, recent study by Dogui et al  showed consistency between the aortic distensibility and PWV with respect to the Bramwell-Hill model . However, the two methods are expected to disagree on muscular arteries (e.g. femoral artery) that are better characterized as viscoelastic material where the modulus of the arterial wall has stronger dependency on the oscillatory nature of the arterial pressure fluctuation. Since measurement of distensibility fails to capture the age-related effect (possibly endothelial dysfunction, as well) we suggest the wave-front velocity based method to be better suited for assessing pathophysiology of vascular aging and atherosclerosis of muscular arteries. However, addressing these questions will require comparative studies in larger cohorts of healthy subjects and patients with subclinical atherosclerotic disease.
The work was supported by the grants NIH R21 HL088182, NIH RC1-HL099861 and R01 HL109545.
- O'Rourke MF, Nichols WW: Aortic diameter, aortic stiffness, and wave reflection increase with age and isolated systolic hypertension. Hypertension. 2005, 45 (4): 652-658. 10.1161/01.HYP.0000153793.84859.b8.View ArticlePubMedGoogle Scholar
- Asmar R, Benetos A, Topouchian J, Laurent P, Pannier B, Brisac AM, Target R, Levy BI: Assessment of arterial distensibility by automatic pulse wave velocity measurement. Validation and clinical application studies. Hypertension. 1995, 26 (3): 485-490.View ArticlePubMedGoogle Scholar
- Wilkinson IB, Fuchs SA, Jansen IM, Spratt JC, Murray GD, Cockcroft JR, Webb DJ: Reproducibility of pulse wave velocity and augmentation index measured by pulse wave analysis. J Hypertens. 1998, 16 (12 Pt 2): 2079-2084.View ArticlePubMedGoogle Scholar
- Sutton-Tyrrell K, Mackey RH, Holubkov R, Vaitkevicius PV, Spurgeon HA, Lakatta EG: Measurement variation of aortic pulse wave velocity in the elderly. Am J Hypertens. 2001, 14 (5 Pt 1): 463-468.View ArticlePubMedGoogle Scholar
- Wenn CM, Newman DL: Arterial tortuosity. Australasian physical & engineering sciences in medicine/supported by the Australasian College of Physical Scientists in Medicine and the Australasian Association of Physical Sciences in Medicine. 1990, 13 (2): 67-70.Google Scholar
- Dogui A, Kachenoura N, Frouin F, Lefort M, De Cesare A, Mousseaux E, Herment A: Consistency of aortic distensibility and pulse wave velocity estimates with respect to the Bramwell-Hill theoretical model: a cardiovascular magnetic resonance study. J Cardiovasc Magn Reson. 2011, 13: 11-10.1186/1532-429X-13-11.PubMed CentralView ArticlePubMedGoogle Scholar
- Mohiaddin RH, Firmin DN, Longmore DB: Age-related changes of human aortic flow wave velocity measured noninvasively by magnetic resonance imaging. J Appl Physiol. 1993, 74 (1): 492-497.PubMedGoogle Scholar
- Rogers WJ, Hu YL, Coast D, Vido DA, Kramer CM, Pyeritz RE, Reichek N: Age-associated changes in regional aortic pulse wave velocity. Journal of the American College of Cardiology. 2001, 38 (4): 1123-1129. 10.1016/S0735-1097(01)01504-2.View ArticlePubMedGoogle Scholar
- Redheuil A, Yu WC, Wu CO, Mousseaux E, de Cesare A, Yan R, Kachenoura N, Bluemke D, Lima JA: Reduced ascending aortic strain and distensibility: earliest manifestations of vascular aging in humans. Hypertension. 2010, 55 (2): 319-326. 10.1161/HYPERTENSIONAHA.109.141275.PubMed CentralView ArticlePubMedGoogle Scholar
- Westenberg JJ, de Roos A, Grotenhuis HB, Steendijk P, Hendriksen D, van den Boogaard PJ, van der Geest RJ, Bax JJ, Jukema JW, Reiber JH: Improved aortic pulse wave velocity assessment from multislice two-directional in-plane velocity-encoded magnetic resonance imaging. J Magn Reson Imaging. 2010, 32 (5): 1086-1094. 10.1002/jmri.22359.View ArticlePubMedGoogle Scholar
- Hardy CJ, Bolster BD, McVeigh ER, Adams WJ, Zerhouni EA: A one-dimensional velocity technique for NMR measurement of aortic distensibility. Magn Reson Med. 1994, 31 (5): 513-520. 10.1002/mrm.1910310507.PubMed CentralView ArticlePubMedGoogle Scholar
- Hardy CJ, Bolster BD, McVeigh ER, Iben IE, Zerhouni EA: Pencil excitation with interleaved fourier velocity encoding: NMR measurement of aortic distensibility. Magn Reson Med. 1996, 35 (6): 814-819. 10.1002/mrm.1910350605.PubMed CentralView ArticlePubMedGoogle Scholar
- Macgowan CK, Henkelman RM, Wood ML: Pulse-wave velocity measured in one heartbeat using MR tagging. Magn Reson Med. 2002, 48 (1): 115-121. 10.1002/mrm.10177.View ArticlePubMedGoogle Scholar
- Hickson SS, Butlin M, Graves M, Taviani V, Avolio AP, McEniery CM, Wilkinson IB: The relationship of age with regional aortic stiffness and diameter. JACC Cardiovasc Imaging. 2010, 3 (12): 1247-1255. 10.1016/j.jcmg.2010.09.016.View ArticlePubMedGoogle Scholar
- Kawasaki T, Sasayama S, Yagi S, Asakawa T, Hirai T: Non-invasive assessment of the age related changes in stiffness of major branches of the human arteries. Cardiovasc Res. 1987, 21 (9): 678-687. 10.1093/cvr/21.9.678.View ArticlePubMedGoogle Scholar
- van der Heijden-Spek JJ, Staessen JA, Fagard RH, Hoeks AP, Boudier HA, van Bortel LM: Effect of age on brachial artery wall properties differs from the aorta and is gender dependent: a population study. Hypertension. 2000, 35 (2): 637-642.View ArticlePubMedGoogle Scholar
- Ahlgren AR, Astrand H, Sandgren T, Vernersson E, Sonesson B, Lanne T: Dynamic behaviour of the common femoral artery: age and gender of minor importance. Ultrasound Med Biol. 2001, 27 (2): 181-188. 10.1016/S0301-5629(00)00339-2.View ArticlePubMedGoogle Scholar
- Bjarnegard N, Lanne T: Arterial properties along the upper arm in humans: age-related effects and the consequence of anatomical location. J Appl Physiol. 2010, 108 (1): 34-38. 10.1152/japplphysiol.00479.2009.View ArticlePubMedGoogle Scholar
- Bortolotto LA, Hanon O, Franconi G, Boutouyrie P, Legrain S, Girerd X: The aging process modifies the distensibility of elastic but not muscular arteries. Hypertension. 1999, 34 (4 Pt 2): 889-892.View ArticlePubMedGoogle Scholar
- O'Rourke MF: Vascular impedance in studies of arterial and cardiac function. Physiol Rev. 1982, 62 (2): 570-623.PubMedGoogle Scholar
- Learoyd BM, Taylor MG: Alterations with age in the viscoelastic properties of human arterial walls. Circ Res. 1966, 18 (3): 278-292.View ArticlePubMedGoogle Scholar
- Langham MC, Li C, Magland JF, Wehrli FW: Nontriggered MRI quantification of aortic pulse-wave velocity. Magn Reson Med. 2011, 65 (3): 750-755. 10.1002/mrm.22651.PubMed CentralView ArticlePubMedGoogle Scholar
- Laurent S, Cockcroft J, Van Bortel L, Boutouyrie P, Giannattasio C, Hayoz D, Pannier B, Vlachopoulos C, Wilkinson I, Struijker-Boudier H: Expert consensus document on arterial stiffness: methodological issues and clinical applications. Eur Heart J. 2006, 27 (21): 2588-2605. 10.1093/eurheartj/ehl254.View ArticlePubMedGoogle Scholar
- Vulliemoz S, Stergiopulos N, Meuli R: Estimation of local aortic elastic properties with MRI. Magn Reson Med. 2002, 47 (4): 649-654. 10.1002/mrm.10100.View ArticlePubMedGoogle Scholar
- Taviani V, Hickson SS, Hardy CJ, McEniery CM, Patterson AJ, Gillard JH, Wilkinson IB, Graves MJ: Age-related changes of regional pulse wave velocity in the descending aorta using Fourier velocity encoded M-mode. Magn Reson Med. 2011, 65 (1): 261-268.View ArticlePubMedGoogle Scholar
- Orlandi A, Bochaton-Piallat ML, Gabbiani G, Spagnoli LG: Aging, smooth muscle cells and vascular pathobiology: implications for atherosclerosis. Atherosclerosis. 2006, 188 (2): 221-230. 10.1016/j.atherosclerosis.2006.01.018.View ArticlePubMedGoogle Scholar
- Lantelme P, Mestre C, Lievre M, Gressard A, Milon H: Heart rate: an important confounder of pulse wave velocity assessment. Hypertension. 2002, 39 (6): 1083-1087. 10.1161/01.HYP.0000019132.41066.95.View ArticlePubMedGoogle Scholar
- Albaladejo P, Copie X, Boutouyrie P, Laloux B, Declere AD, Smulyan H, Benetos A: Heart rate, arterial stiffness, and wave reflections in paced patients. Hypertension. 2001, 38 (4): 949-952. 10.1161/hy1001.096210.View ArticlePubMedGoogle Scholar
- Mangoni AA, Mircoli L, Giannattasio C, Ferrari AU, Mancia G: Heart rate-dependence of arterial distensibility in vivo. J Hypertens. 1996, 14 (7): 897-901. 10.1097/00004872-199607000-00013.View ArticlePubMedGoogle Scholar
- Bramwell JC, Hill AV: The Velocity of the Pulse Wave in Man. Proceedings of the Royal Society of London Series B, Containing Papers of a Biological Character. 1922, 93 (652): 298-306. 10.1098/rspb.1922.0022.View ArticleGoogle Scholar
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