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Serial diffusion tensor MRI and tractography of the mouse heart in-vivo: impact of ischemia on myocardial microstructure
Journal of Cardiovascular Magnetic Resonance volume 13, Article number: O28 (2011)
To perform serial in-vivo diffusion tensor MRI (DTI) and tractography of the mouse heart and characterize the impact of ischemia on myocardial microstructure.
Diffusion tensor MRI (DTI) has been used to investigate infarct healing and remodeling in several species. Tractography of the myocardium has also recently been reported. However, these prior studies were conducted ex-vivo on excised hearts. Here, we use in-vivo DTI of the mouse heart to follow microstructural changes in the myocardium in response to ischemia. Mean diffusivity (MD) and fractional anisotropy (FA) were measured 24 hours and 2-3 weeks after ischemia-reperfusion. In addition, 3D diffusion MRI tractography of the mice hearts was performed in-vivo.
Material and methods
11 mice were used: Six as normal controls and five exposed to 35min left coronary artery ligation followed by reperfusion. In vivo DTI was performed 24 hours and 2-3 weeks post-injury on a 9.4T scanner (Biospin, Bruker) with a 150 Gauss/cm gradient. The in- vivo DTI sequence was based on a fat-suppressed single-shot spin echo EPI sequence with motion-compensated bipolar diffusion-encoding gradients on either side of the 180° RF pulse. Imaging parameters included: FOV: 2.0 x 2.0 cm2, matrix: 70 x 70 (padded to 128 x 128), TR/TE: 2000/13.5 ms, b-value 500 - 700 sec/mm2. 3D parameters were similar but with an isotropic resolution of 280 um.
A significant (* p < 0.05) increase in MD and decrease in FA were seen in mice 24 hours after ischemia-reperfusion (Figure 1). Significant differences in MD and FA were also seen between the ischemic mice at 24hrs and 2-3 weeks of follow-up (Figure 1). In-vivo tractography produced tractograms of similar quality to those previously acquired ex-vivo, with the characteristic crossing myofiber helices well seen in normal mice (Figure 2A). Myofiber architecture was markedly perturbed in the ischemic mice (Figure 2B-C). A large increase in MD (>1.3 x 10-3 mm2/sec) at 24 hours correlated strongly with a severe loss of myofiber architecture (Figure 2B-C).
Combined in-vivo DTI and tractography of the myocardium is presented for the first time. The technique shows that MD increases and FA decreases in acute ischemia, and thereafter return towards baseline as the myocardium heals. The loss of myofiber architecture in acute ischemia is variable, but is marked when MD is significantly elevated. Ongoing use of these techniques in-vivo has the potential to become an extremely powerful tool in cardiovascular MRI.
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Open Access This article is published under license to BioMed Central Ltd. This is an Open Access article is distributed under the terms of the Creative Commons Attribution 2.0 International License (https://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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Huang, S., Mekkaoui, C., Chen, H.H. et al. Serial diffusion tensor MRI and tractography of the mouse heart in-vivo: impact of ischemia on myocardial microstructure. J Cardiovasc Magn Reson 13 (Suppl 1), O28 (2011). https://doi.org/10.1186/1532-429X-13-S1-O28
- Fractional Anisotropy
- Mean Diffusivity
- Mouse Heart
- Acute Ischemia
- Coronary Artery Ligation