Manganese kinetics demonstrated a double contrast in acute but not in chronic infarction in a mouse model of myocardial ischemia reperfusion
© Delattre et al; licensee BioMed Central Ltd. 2011
Published: 2 February 2011
In this study we investigated whether Manganese (Mn2+) wash-in kinetics can add new information regarding a myocardial infarct characterization in particular if it can differentiate an acute infarct from a chronic scar.
Manganese (Mn2+) is considered as a specific MRI contrast agent that enters viable cardiomyocytes through calcium pathways. Compared to extracellular Gadolinium based contrast agents, it has the potential to assess cell viability and has already shown its ability to accurately measure infarct size with late enhancement imaging. So far, only information from the wash out phase after recirculation has been used for the detection and characterization of myocardial infarct.
In this study, we used a model of 60min ischemia followed by reperfusion on C57/BL6 mice. MRI exam was performed on a clinical 3T scanner, either 24 hours (n = 10) or 8 days (n =12) after reperfusion (acute and chronic infarct respectively). Mn2+-induced signal intensity (SI) kinetics were measured into three distinct areas, remote, infarction and left ventricular blood pool and compared to ex vivo TTC and Masson’s trichrome.
In addition to its ability to depict accurately the infarcted area at late enhancement, Mn2+ is also able to discriminate acute versus chronic injury by the observation of double-contrast wash-in kinetics in a mouse model of ischemia reperfusion.
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