PPACK and Bivalirudin nanoparticles enable simultaneous imaging and potent inhibition of acute clotting
© Myerson et al; licensee BioMed Central Ltd. 2012
Published: 1 February 2012
Perfluorocarbon nanoparticles functionalized for thombin inhibition with Bivalirudin or PPACK were tested as an inhibitor of thrombin in vitro and in acute thrombosis models. The particles significantly inhibited occlusive arterial thrombi. The particles also manifested binding providing magnetic resonance contrast highlighting thrombi.
Acute thrombosis is currently addressed with a cocktail of anticoagulants, antiplatelet agents, and thrombolytics. Optimization of antithrombotics for clinical use remains a significant research challenge. Thrombin inhibitors represent a promising class of anticoagulant. Imaging contrast agents specific to thrombi would represent a boon to clinical intervention, enabling monitoring of thrombosis. In recent work, a thrombin-inhibiting perfluorocarbon nanoparticle (PFC NP), functionalized by the direct thrombin inhibitor, PPACK (Phe(D)-Pro-Arg-Chloromethylketone), was presented as a prototype for novel class of targeted antithrombotic. Here, an NP functionalized with Bivalirudin (BVR), was compared to BVR and the PPACK NP. Both particles were examined as agents for inhibition and imaging of acute thrombosis.
PPACK or BVR were covalently attached to PFC NPs. Optical assay verified that PPACK and BVR selectivity and activity against thrombin was not diminished on the NPs. In vivo activity was assessed for PPACK NPs, PPACK, BVR, BVR NPs, heparin, non-functionalized NPs, or saline in C57BL6 mice subjected to laser injury of the carotid artery. Time to thrombotic occlusion of the injured artery was assessed via Doppler flow measurement. Selected arteries were excised to assess NP retention via 19F MR at 12T. The laser injury arterial thrombosis model was applied in cholesterol-fed rabbits to demonstrate 3T MRI of thrombosis following administration of PPACK NPs. 19F MRI of the arterial segment at 12T enabled mapping of the thrombus.
Anticoagulant PFC NPs were designed as new antithrombotics with intrinsic magnetic resonance contrast, concentrated therapeutic impact conferred by a thrombin-specific particle surface, and well-defined pharmacokinetics controlled by the particle itself. As potent agents that can enhance the therapeutic performance of different thrombin inhibitors, these NPs are promising agents. Further clinical potential is conferred on the thrombin-inhibiting PFC NP by its ability to guide further intervention by specifically localizing magnetic resonance contrast to sites of thrombosis.
This work was supported by National Institutes of Health Grants R01 HL073646 and U54 CA119342 to Samuel Wickline, R01 NS059302 to Gregory Lanza, and R01 HL55520 to Douglas Tollefsen.
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