- Poster presentation
- Open Access
Thrombus-specific manganese-based “nanobialys” for MR molecular imaging of ruptured plaque
© Pan et al; licensee BioMed Central Ltd. 2012
- Published: 1 February 2012
- Atherosclerotic Plaque
- Molecular Imaging
- Fibrin Clot
- Imaging Contrast Agent
- Severe Renal Disease
Mathematical modeling studies have suggested that nonspherical, disc-shaped nanoparticles may have optimal intravascular flow and homing characteristics. In this study, we report the development of a fibrin-specific high-relaxivity bialy-shaped polymeric nanoparticle using porphyrin-chelated manganese. We anticipate that this agent would be highly effective for molecular imaging of microthrombi in ruptured atherosclerotic plaques.
Detection of microthrombi within fissures of vulnerable atherosclerotic plaques requires a sensitive molecular imaging contrast agent. Moreover, recent reports based on mathematical modeling suggest that nonspherical, disc-shaped nanoparticles could have improved intravascular flow characteristics, which may improve ligand-directed targeting. In light of the concern surrounding the use of gadolinium in patients with severe renal disease, the goal of this research was to develop a nonspherical fibrin-targeted manganese-based molecular imaging agent.
A new class of manganese (III)-labeled, a toroidal-shaped, vascularly-constrained nanoparticles, “nano-bialys (MnNBs)”, was designed, synthesized, physically characterized, and evaluated for MR properties. Single slice inversion recovery and multi-echo spin echo sequences were used to calculate the ionic (per metal) and particulate (per particle) relaxivities from 7 serial dilutions of nanobialys at 1.5T and 25°C. Fibrin clots supported on silk suture suspended in PBS were targeted with MnNB or control (non-paramagnetic) NB to the fibrin clots with avidin-biotin interactions and fibrin-specific antibodies (NIB5F3). Magnetic resonance images (3T) of the clots were acquired using T1-weighted gradient echo techniques.
Fibrin-specific MnNBs are a novel, high relaxivity, non-gadolinium, molecular imaging agent that offers a sensitive noninvasive MR imaging approach for diagnosis of ruptured atherosclerotic plaques.
This article is published under license to BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.