- Poster presentation
- Open Access
Multimodal MRI in the course of cardiac wound healing after myocardial infarction
© Bönner et al; licensee BioMed Central Ltd. 2012
- Published: 1 February 2012
- Myocardial Tissue
- Cine Imaging
- Late Enhancement
- Coronary Ligation
- Wall Movement Analysis
Myocardial tissue undergoes various adaptive processes in the course of healing after myocardial infarction including infiltration of immunocompetent cells. Assessment of myocardial infarction and subsequent healing with contrast-enhanced and T2-weighted MRI holds the advantage of non-invasive localisation and characterisation of myocardial lesions, determination of their functional relevance and monitoring oedema formation and resorbtion in myocardial tissue. A quantitative method to determine immune cell migration into myocardial tissue as consequence of myocardial infarction is provided by 19F MRI after intravenous application of emulsified perfluorocarbons (PFCs).
For induction of myocardial infarction C57Bl/6 mice underwent 50 min of coronary ligation with subsequent reperfusion. MRI measurements were carried out with a DRX 9.4 T NMR spectrometer (Bruker, Rheinstetten, Germany). Cine imaging for cardiac function analysis was performed using a respiration- and ECG-gated gradient echo sequence. For contrast-enhanced measurements a T1 weighted gradient echo sequence was used to detect myocardial necrosis after contrast agent application (Gadolinium DTPA). T2 maps were calculated from gated multi spin echo images. All measurements were performed at baseline, 1, 7, 14 and 28 days after I/R. Infiltration of immune cells was measured by 19F MRI (multislice RARE sequence, factor 64) at days 3 and 7 after I/R and intravenous injection of 500µl PFCs 24 hrs prior to MRI.
In summary, multimodal MRI after I/R provides complementary information and therefore holds promise for serial analysis of myocardial tissue characteristics. Since all measurements can be performed with one setup, no repositioning of the animals is required enabling an exact overlay of identical myocardial regions. The combination of cine imaging, late enhancement and T2-mapping techniques with 19F MRI enables a comprehensive analysis of functional, morphological and inflammatory properties of the healing myocardium after infarction in vivo.
This article is published under license to BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.