- Poster presentation
- Open Access
Manganese-enhanced MRI in the evaluation of cell-based therapy for myocardial restoration
© Kim et al; licensee BioMed Central Ltd. 2012
- Published: 1 February 2012
- Leave Anterior Descend
- Viable Myocardium
- Stem Cell Therapy
- Bioluminescence Image
- Myocardial Salvage
To date, the underlying mechanism responsible for the restoration of the injured myocardium following transplantation of stem cells has not been clearly identified. Three major hypotheses have been previously proposed: cardiac differentiation of transplanted cells (de novo myocardial regeneration), paracrine effect on existing myocardium (myocardial salvage) or recruitment of cardiac progenitor cells. Manganese-enhanced MRI (MEMRI) has recently been published as a reliable method of imaging viable myocardium (Dash et al 2011). Utilizing MEMRI, we evaluated the changes in the viability of the injured myocardium to further investigate the underlying mechanism of functional restoration using stem cell therapy.
Thirteen Fox Chase SCID Beige mice were subjected to permanent left anterior descending (LAD) ligation to create the mouse myocardial infarction model. 2.5 x 10^5 reporter-gene transduced embryonic stem cells (ESC-RGs) containing firefly luciferase (fluc) were subsequently transplanted into the intra-infarct region in eleven mice. Two mice were injected with normal saline into the intra-infarct region to serve as controls. 3T cardiac MRI was performed weekly for 4 weeks following LAD ligation and ESC-RGs transplantation to obtain LVEF measurements and MEMRI images. Additionally, bioluminescence images (BLI) were obtained weekly utilizing the transduced fluc gene to demonstrate persistent viability of the ESC-RGs. At selected time points (weeks 2, 3 and 4), the hearts were explanted, sectioned along the short axis plane and processed for H&E staining. The H&E stained slides provided histological correlation of MEMRI and bioluminescence images.
This study demonstrates the functional improvement in ESC-RG transplanted mice. In addition, MEMRI shows a significant increase in viable myocardium in ESC-RG transplanted hearts, indicating myocardial restoration. This finding may support the hypothesis that functional improvement with stem cell therapy may be due to myocardial salvage.
T32 CVIS Grant under National Institute of Biomedical Imaging and Bioengineering.
This article is published under license to BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.