- Poster presentation
- Open Access
Cine interleaved sequences enabled imaging of mice on clinical 3T MRI and analysis of their cardiac function after myocardial infarction
© Belin et al; licensee BioMed Central Ltd. 2012
- Published: 1 February 2012
- Myocardial Infarct
- Ejection Fraction
- Cardiac Function
- Fourier Coefficient
- Left Coronary Artery
With the poor availability of small animal dedicated MRI, it is of great interest and challenge to use clinical MRI to image and analyze the cardiac function of mice. This would of course be advantageous for translational research and also enable the use of up-to-date sequences already implemented in clinical routine. The aim is to study the time evolution of cardiac function in mice with a myocardial infarct on a clinical MRI.
C57BL/6 mice (n=4) were submitted in vivo to left coronary artery permanent ligature and compared and with compared with non-operated mice (n=4). The mice were imaged on a clinical Siemens 3T MRI using an “interleaved” sequence constructed from an ECG-triggered turboflash cine sequence, combining two acquisitions shifted in time yielding an effective time resolution of 6.8 ms and 20-26 phases per heart beat with following parameters: FOV 111 mm, in-plane resolution 257 μm, slice thickness 1 mm, TE/TR 6.2/13.5 ms, flip angle 30°. A soft-thresholding of the temporal Fourier coefficients was used to further denoise the images. The mice were scanned at 24h and 22 days after coronary ligation.
Cardiac properties of the mice
We demonstrated a robust protocol to study cardiac functions in mice with a myocardial infarct using a clinical 3T MRI. This methodology has a strong potential to study the effect of treatment in rodents.
This work was partially supported by the Swiss National Science Foundation (grant PP00P2-123438).
This article is published under license to BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.