- Position statement
- Open Access
Myocardial T1 mapping and extracellular volume quantification: a Society for Cardiovascular Magnetic Resonance (SCMR) and CMR Working Group of the European Society of Cardiology consensus statement
- James C Moon†1, 2Email author,
- Daniel R Messroghli†3,
- Peter Kellman4,
- Stefan K Piechnik5,
- Matthew D Robson5,
- Martin Ugander6,
- Peter D Gatehouse7,
- Andrew E Arai4,
- Matthias G Friedrich8,
- Stefan Neubauer5,
- Jeanette Schulz-Menger9, 10 and
- Erik B Schelbert11
© Moon et al.; licensee BioMed Central Ltd. 2013
- Received: 4 October 2013
- Accepted: 4 October 2013
- Published: 14 October 2013
Rapid innovations in cardiovascular magnetic resonance (CMR) now permit the routine acquisition of quantitative measures of myocardial and blood T1 which are key tissue characteristics. These capabilities introduce a new frontier in cardiology, enabling the practitioner/investigator to quantify biologically important myocardial properties that otherwise can be difficult to ascertain clinically. CMR may be able to track biologically important changes in the myocardium by: a) native T1 that reflects myocardial disease involving the myocyte and interstitium without use of gadolinium based contrast agents (GBCA), or b) the extracellular volume fraction (ECV)–a direct GBCA-based measurement of the size of the extracellular space, reflecting interstitial disease. The latter technique attempts to dichotomize the myocardium into its cellular and interstitial components with estimates expressed as volume fractions. This document provides recommendations for clinical and research T1 and ECV measurement, based on published evidence when available and expert consensus when not. We address site preparation, scan type, scan planning and acquisition, quality control, visualisation and analysis, technical development. We also address controversies in the field. While ECV and native T1 mapping appear destined to affect clinical decision making, they lack multi-centre application and face significant challenges, which demand a community-wide approach among stakeholders. At present, ECV and native T1 mapping appear sufficiently robust for many diseases; yet more research is required before a large-scale application for clinical decision-making can be recommended.
- Cardiovascular Magnetic Resonance
- Late Gadolinium Enhancement
- Fabry Disease
- Gadolinium Base Contrast Agent
- Post Contrast
The “T1 mapping development group” was informally founded October 2010. Its mission statement is “to facilitate the path of T1 mapping and ECV quantification into clinical practice”. It is aimed at academics, clinicians, pharmaceutical companies, equipment manufacturers and software developers who have a strong interest in the quantification of diffuse myocardial processes by cardiovascular magnetic resonance (CMR) with T1 mapping as their primary or core activity, and who wish to coordinate activity to the goal of being able to change therapy using these endpoints. Minutes from prior regular meetings are available . The nucleus of the group and senior advisors documented in the introduction have a range of technical and clinical expertise and a broad geographical base. Together, they have provided many of the key innovations in the field. The group is now affiliated with the Society for Cardiovascular Magnetic Resonance (SCMR) and the CMR Working Group of the European Society of Cardiology.
Rapid innovations in CMR now permit the routine acquisition of quantitative measures of myocardial and blood T1 which are key tissue characteristics. T1 quantification requires the acquisition of multiple images to derive the T1 recovery curve which is governed by the exponential time constant for MR longitudinal relaxation, T1. This parameter can be displayed as a pixelwise “T1 map” whereby an estimate of T1 is encoded in the intensity of each pixel [2, 3]. Its quantitative nature permits establishing normal T1 ranges, and T1 values can be assigned colors to simplify visual interpretation.
This capability introduces a new frontier in cardiology, enabling the practitioner/investigator to quantify biologically important properties of both regional and global myocardium independent of function. Historically, diffuse myocardial disease has been difficult to measure or even appreciate noninvasively. This advance is important, because focal and diffuse changes may directly reflect pathophysiologic processes across the disease spectrum from preclinical to end-stage disease. CMR may be able to track biologically important biologically important changes in the myocardium by: a) native (noncontrast) T1 that reflects myocardial disease involving the myocyte and interstitium without use of gadolinium based contrast agents (GBCA), or b) the extracellular volume fraction (ECV) after a GBCA–a direct measurement of the size of the extracellular space, reflecting interstitial disease. The latter technique attempts to dichotomize the myocardium into its cellular and interstitial components with estimates expressed as volume fractions. The concept of employing extracellular agents to quantify the interstitial space has been exploited by investigators over many decades [4–7]. Advances in T1 measurement now permit routine noninvasive measurement of ECV.
This document provides recommendations for clinical and research T1 and ECV measurement, based on published evidence when available and expert consensus when not. We recognize a priori that multiple methodologies for T1 measures do and should exist, with continued evolution and residual imperfections. Furthermore, different vendor implementations of the same biomarker test may have different normal ranges. However, the rapid progress in the field renders it sufficiently mature to warrant recommendations. We make analogy to another key cardiac imaging biomarker, the left ventricular ejection fraction (LVEF), where measurement variations persist within and across modalities yet the yield of biological information is sufficient to diagnose, guide and monitor treatment and predict outcome. In fact, all imaging and non imaging biomarkers share some degree of error inherent in their measurement which is implicit in their “signal to noise” ratios and their coefficients of variation.
Native (Noncontrast) T1
Native (noncontrast) T1 measures of myocardium permit noninvasive detection of biologically important processes which promise to improve diagnosis, measures of disease severity, and potentially prognosis. Native T1 changes can detect pathologically important processes related to excess water in oedema [2, 8, 9], protein deposition [10, 11], and other T1-altering substances such as lipid [12, 13] or iron (hemorrhage, siderosis) , without the need for a GBCA. In addition native T1 techniques need not exclude patients with severe renal dysfunction. Alterations of myocardial native T1 can therefore signal both cardiac diseases (acute coronary syndromes, infarction, myocarditis, diffuse fibrosis causes (all high T1)) , and systemic disease such as (cardiac amyloid (high T1))  Anderson-Fabry disease (low T1)  and siderosis (low T1). When combined in a clinical scan protocol, early evidence suggests that native T1 mapping can reveal pathology such as area at risk in acute coronary syndromes [2, 8, 9, 17], hitherto unsuspected pathologies (global myocarditis without LGE) and preclinical disease or unsuspected cardiac involvement (iron, Fabry disease, amyloid) [10, 12, 18].
The ECV technique introduces a potentially important new method to examine the myocardium because it is sensitive to the distribution of the LV myocardium into its cellular (dominated by myocyte mass) and extracellular interstitial (extracellular matrix (ECM) in the interstitium) compartments. Alterations in these compartments occur from different physiologic and pathophysiologic biologic processes .
Early data indicate that ECV measures appear to be as prognostically important as LVEF [20, 21] which underscores the biologic importance of the interstitium. This myocyte-ECM expansion dichotomy may have important implications for identifying distinct therapeutic targets: i.e., the fibroblast versus the myocyte. This issue is especially important in heart failure where over 20 trials failed to identify therapeutic targets . Furthermore, in heart failure with preserved systolic function, there are no evidence based therapies to reduce hospitalization or mortality. The extent to which primary ECM expansion from fibroblast activation drives myocyte dysfunction or the extent to which primary myocyte disease leads to ECM expansion in HF remains incompletely understood, but now the cardiology community has a developing and promising means to quantify expansion of the interstitium.
In the absence of amyloid or oedema , expansion of the myocardial collagen volume fraction is responsible for most ECM expansion  which culminates in mechanical [25–27], electrical [28–31], and vasomotor dysfunction , which are key elements of cardiac vulnerability . ECM expansion can diminish tolerance to ischemic insults [34–36]. Other investigators have reported “vulnerable interstitium” in sudden cardiac death victims , and have described band-like fibrosis in myocardium resembling hepatic cirrhosis . Thus, just like other organs, fibrosis in the myocardium is associated with cardiac dysfunction . Fibrosis is associated with a number of conditions [39, 40] and is considered to represent a final common pathway of myocardial disease from a variety of insults.
While late gadolinium enhancement (LGE) undoubtedly provides important diagnostic and prognostic information [41–47], T1 mapping and ECV may have an advantage over LGE for quantifying the degree of ECM or interstitial expansion. LGE is less suitable for quantifying extent of ECM expansion [48–54] resulting from pathologies other than myocardial infarction where the differences between normal and affected myocardium are less distinct. LGE validation data for this purpose are lacking. Spatial variation of myocardial fibrosis is the key feature that renders it potentially detectable on an LGE image . In ischemic cardiomyopathy, one small study examining 10 explanted hearts reported that the majority of the total collagen content could be distributed diffusely rather than focally . Such a diffuse distribution of collagen content would render its accurate quantification nearly impossible with LGE. ECV can detect early fibrosis changes not always detectable by LGE [20, 49–51, 55–58]. The association with outcomes appears stronger for ECV compared to LGE [20, 21]. Automated parametric ECV maps are an exciting development that may facilitate rapid ECV measurement and potentially catalyze the field .
To streamline the field and increase its accessibility, below are a list of recommended terms and their definitions
Longitudinal relaxation time (T1) values of a given tissue when no contrast agent has been applied. “Native T1” is preferred over other terms such as “pre-contrast T1” or “non-contrast T1”. If a paper is unambiguous (no contrast use, no measurement of other tissues), after initial use, native myocardial T1 can be abbreviated to simply T1.
A CMR method providing a parametric map whereby the T1 value is encoded in each pixel. T1 maps arise from a series of co-registered images acquired at different times of T1 recovery, typically following a magnetisation preparation by inversion or saturation. Raw images used for T1 mapping need to be acquired at identical times in the cardiac cycle. CMR methods allowing for T1 estimation from ROIs drawn in raw images of different parts of the cardiac cycle (i.e. not allowing pixel-based T1 analysis) should be referred to as T1 measurements.
ECV or myocardial ECV
The extracellular volume (ECV) of the myocardium reflects the volume fraction of heart tissue that is not taken by cells. This includes the intracapillary plasma volume–a significant compartment in some organs like the liver. ECV should be preferred over other terms such as “volume of distribution”. ECV does not account for regression in capillary density or other microvasculature throughout the myocardium that may be associated with adverse remodelling [60–62]. Yet, such decreases in the myocardial vasculature would only mask differences in ECM expansion that ECV attempts to measure. ECV maps can also be generated on a pixel-wise basis if native and post contrast T1 images are coregistered, quantified, and adjusted for the hematocrit .
ICV or myocardial intracellular volume
The residual of ECV (i.e., 1-ECV = ICV) representing the total tissue volume inaccessible to the GBCA molecules themselves–i.e., behind cell membranes, the composite of all cells (mostly myocyte mass, but also red blood cells, fibroblasts, macrophages, etc.). However, ICV estimates are subject to the same biases inherent in ECV estimates.
Neither T1 mapping nor ECV directly measure the extracellular matrix or detect other important ECM qualities, such as the degree of crosslinking and post translational modification. Rather, ECV measures the space the ECM occupies which is a useful surrogate. ECV has robust histological validation as an ECM measurement which correlates with the collagen volume fraction [24, 54, 63]. This advance is important because myocardial fibrosis is ubiquitous and associated with myocardial remodeling [19, 37, 39, 40, 46, 64, 65]. In the absence of amyloidosis, other forms of infiltrative disease, or clinical conditions that would create myocardial edema, and acknowledging the other components of extracellular matrix , ECV is a CMR biomarker for myocardial fibrosis. We suggest avoiding use of the term “scar” due to its potential for confusion. It is not clear whether “scar” refers to: necrosis, apoptosis, or fibrosis; an ischemic etiology or non-ischemic etiology; focal or diffuse fibrosis.
- 1.Site preparation
Establish site normative values for the particular set-up (vendor/field strength/magnet/contrast regime/sequence variant and patient population (age/gender)).
Use a validated sequence with tightly controlled protocol for the duration of the study.
Specify the field strength and provide a method name prominently with details about the specific pulse sequence employed to measure T1 which can affect the range of values encountered in healthy volunteers and the sensitivity to the disease process.
- 2.Scan types
For studies involving GBCA, the preferred outputs are native T1 and ECV, and not partition coefficient and post contrast T1 in isolation.
Haematocrit for ECV calculation should be measured contemporaneously with the CMR study.
A “bolus only” approach to ECV measurement is sufficient for most myocardial ECV applications.
GBCA should not be a protein bound variant for ECV measurement.
- 3.Scan planning and acquisition
Through plane partial volume averaging should be minimized by optimal slice orientation relative to the tissue (i.e., structures should be orthogonal to the imaging plane to minimize obliquity)
Ensure proper adjustment of shim and center frequency to minimize off resonance
Native and post contrast T1 maps should be acquired using the same slice prescription parameters and the same cardiac phase
- 4.Quality control
Quality measure maps such as “goodness of fit” or parameter error maps [9, 18, 67] should be included in the interpretation to assess the quality of acquired data. Preferably this should be performed during scanning to allow an immediate repeat of suboptimal measurements.
Multiple (≥2) acquisitions in different slice orientations are recommended to add diagnostic confidence.
- 5.Visualisation and analysis
T1 and ECV maps may be displayed in color (or grayscale) with appropriate scale to maximize differentiation from normal and this scale should be kept the same within a study.
Measurements from regions of interest should minimize in plane and through plane (obliquity) partial volume effects.
Regions of interest should have adequate margins of separation from tissue interfaces prone to partial volume averaging such as between myocardium and blood.
The exclusion/inclusion of LGE areas for ECV measures (i.e., myocardial infarction, non-ischemic LGE atypical of myocardial infarction) in T1/ECV quantification should be stated. It is acceptable for regions of interest to exclude infarcts (i.e., remote myocardium) and include non-ischaemic LGE.
- 6.Technical development
New pulse sequences and/or imaging protocols should be validated in phantoms and uniquely named.
T1 and T2 of phantoms should have values expected for the tissue of interest with and without contrast, at the desired field strength.
Evaluations should use a relevant range of heart rates. Any applied corrections, (e.g., heart rate) should be clearly defined.
The proposed approach should be validated against a gold standard such as spin echo relaxometry.
Research studies, should consider, as a guide, the general standards for the reporting of diagnostic accuracy studies (STARD) .
Justification for the consensus recommendations
Site preparationSignificant biases in T1 measurement may depend on the specific method and imaging protocol. Caution must be exercised on relying on phantom or simulation validation which may not account for effects such as T2 relaxation or magnetization transfer (MT) which may be different in-vivo . The “bias” errors may be strongly influenced by imaging parameters such as flip angle, matrix size, slice profile, and numerous other factors. The sensitivity of T1 mapping to imaging parameters is not well quantified, therefore, it is recommended to tightly control the imaging protocol for the duration of the study in order to minimize unintended variation in measured T1 due to these factors. Sequence and software upgrades, even if apparently not affecting T1 mapping (such as new tune up/shims), need to be approached with caution. Normal values for a specific protocol may vary from system to system due to changes in software versions or scanner type, will vary with field strength, and should be measured for each specific configuration. Caution is advised when sharing normative data to ensure that scanner configuration is indeed identical. Normal values should ideally be acquired on normal subject samples (with n ≥ 10, or more if small differences are being sought) representative of the target population distribution. The underlying sequence and imaging protocol should be described or referenced in sufficient detail such that it may be reproduced. A typical scan protocol is supplied, Figure 1.
The relationship between the interstitium and other related parameters, the partition coefficient and isolated post contrast T1, are confounded by clinically relevant conditions arising from variation in the hematocrit, the GBCA dose which varies according to patient weight and GBCA dose conventions (0.1-0.2 mmol/kg) across centres, and the renal clearance after a bolus. Haematocrit varies considerably in large CMR cohorts . To date, ECV appears better associated with patient outcomes [20, 21], compared to the partition coefficient and post contrast T1. Agreement with the collagen volume fraction appears significantly higher for ECV [24, 54, 63] compared to isolated post contrast T1 [70, 71] which does not vary linearly with GBCA concentration. Further study of these issues would be beneficial.
Equilibrium infusion does not give substantially different results to the bolus only approach except at high ECV values where an overestimation bias appears . Whilst longer delays post bolus may have advantages, a 15 minute minimum appears a reasonable compromise balancing with the need for clinical throughput, and is supported by the literature [56, 63, 66]. Although one ECV publication  employed a protein bound GBCA variant, the authors pointed out that such blood protein binding renders two key assumptions invalid: firstly, the assumption of equal extracellular Gd concentrations in blood and tissue at equilibrium; secondly that the Gd relaxivity will be the same (protein binding increases relaxivity by slower molecular tumbling). Use of protein bound GBCA is not currently recommended without a more sophisticated modeling approach and more data.
- 3.Scan planning and acquisition
Shim. The MR reference frequency should be adjusted using a volume region over the heart both within and through the image slice. At higher magnetic fields shimming is particularly important to reduce non-uniformity of the resonance frequency over the heart. Through-slice extent of the volume region used can be important for two reasons: a simple frequency adjustment procedure might not be gated to the same cardiac phase, and motion in the through-plane direction can transport consequences of resonance frequency offsets . Positioning the region of interest near the magnet isocenter is often beneficial .
Breath-hold. Failures of breath-holding will result in the later images not being registered to the initial ones, which causes substantial errors in the calculated maps. Shortened acquisition and motion correction can be beneficial but fundamentally ensuring a good breath-hold is desirable.
Timing of cardiac phase. Gating intervals (e.g., diastole) should not be changed within patients or between patients and normal controls. It is acknowledged that image field of view may need to be changed for patients. As any changes in timing parameters can potentially affect T1 measurements, modification of field-of-view are preferably done without changing acquisition matrix size or allowing any sequence timings to change.
Creation of good quality maps can only be achieved if all of the constituent images are also of good quality. The following need optimization:
It is useful to establish quality measures in order to aid the interpretation and improve the confidence of T1 and ECV maps and associated measurements. In cases of suspected artefacts in T1 or ECV maps, it may be useful to examine the raw magnitude and phase images for image artefacts at the corresponding location. Parametric error maps are useful for quantitatively assessing the validity of T1 or ECV results with the direct estimate of the underlying pixel measurement precision (SD) [18, 67]. The precision may be estimated from standard deviation maps generated for each T1 map . Non random appearance to the error maps showing anatomy may be indicative of uncorrected motion, and thereby raise a cautionary flag in those regions. Motion correction for parametric maps, ideally employing inline automated processing as part of the pulse sequence, is desirable to minimize artefacts related to motion and misregistration, but breath-holding remains desirable. Off-resonance variation across the heart may result in regional variations in apparent T1 . When available, it is useful to have field maps that may be used to ensure that apparent T1 variations are not related to systematic biases rather than true variation in T1 .
Although not as yet evidence based, more than one acquisition is a reasonable approach if clinical decisions are being potentially made on the quantitative results of T1 mapping. This issue is analogous to phase swaps or orthogonal cross-cuts often used for non-ischemic LGE.
Visualisation and analysis
During analysis, significant biases in the value of tissue T1 in a given voxel may arise due to contamination by tissue in adjacent voxels. Regions of interest (ROI) at the interface between myocardium and blood or other tissues such as fat will result in values which represent a complicated distortion of the fundamental curve-fitting assumptions and should be excluded from ROI measurements. The partial volume effect is apparent from both in-plane and through plane sources so care must be exercised to have adequate spatial resolution and slice thickness for the structures of interest, to orient slices judiciously to minimize obliquity during acquisition. During post processing, care must be exercised to draw ROIs sufficiently far from tissue interfaces which represents a key advantage of parametric maps. Criteria used for ROI delineation may have a strong influence on values and should be clearly described. These effects may not only introduce imprecision, but also bias–thinner myocardial walls (eg DCM compared to HCM, females compared to males) include proportionately more blood pool in ROIs, altering T1 (higher native, lower post contrast) and potentially inflating ECV measures.
ECV can be measured in areas of LGE; [75, 76] although for ischaemic heart disease, it may reflect pathology better to dichotomise measurement into “infarct” and “remote”. For non-ischaemic cardiomyopathy, where LGE likely represents the most focal fibrosis in a continuum and where LGE extent is highly thresholding method dependent , LGE may be included in the ECV.
Pulse sequences are continually being developed and refined, but they require initial validation prior to clinical research application. It is important that the evolution is traceable, therefore sequence naming must be unambiguous, and modifications of pulse sequences should utilize version numbers. T1 accuracy and precision should be tested systematically in sets of phantoms that should have T1 and T2 values expected for the tissue of interest with and without contrast, at the desired field strength, with temperature recordings. For example at 1.5 T, consider T1s in the range of T1 = 300-1800 ms, HR = 40-120 bpm, and with two settings of T2 (50 ms and ~180 ms). The proposed approach should be validated against a gold standard such as a series of inversion recovery spin echo acquisitions with long TR and minimal echo trains. The algorithms used for T1 estimation and any applied corrections (e.g., heart rate, systematic bias) should be fully described to allow replication and/or reversing the calculations. The STARD guidelines  do not specifically apply to T1 mapping, but would benefit the field as a guide, so have been included here.
What else influences what we are measuring? T1 mapping promises to improve diagnosis, improve prognosis, and inform mechanisms of disease. Robust T1 measures with high accuracy and precision would support these goals. Yet, there may be trade-offs for various T1 measuring schemes in terms of their accuracy and precision. While accuracy is desirable, precision is especially important to avoid misclassification of individuals and to stratify samples efficiently. Several factors may influence accuracy and precision of T1 measures.
The current methodology for in-vivo measurement and mapping of T1 or ECV assumes a relatively simple model that classifies a voxel as consisting of a single compartment with a homogeneous single value of the parameter T1 or ECV. According to this approximation a mono-exponential may be used to fit the measured response to either inversion or saturation recovery. In fact, we know that biological and molecular structures found in-vivo are more complex and the characteristic time constants will depend on the measurement time scales. Further, the effects such as magnetization transfer (MT), diffusion distance and time, contrast mechanisms, trans-cytolemmal water exchange rate, flow, T2 or T2* relaxation may significantly alter the apparent T1 estimates indicated by any specific method [69, 78–80]. Based on these issues, there is active debate about what influences what we are really measuring, whether some methods are preferable, or whether there are still better approaches. Validation of methods based on phantoms such as agar gel is an important step but does not provide sufficient complexity to answer many of these questions. Despite this uncertainty it has been shown that current methods, if applied carefully, are reproducible  and are valuable tools in clinical research that correlate well with histological gold standards. In addition to the question of surrounding the simplified notion of a single T1 and how it is measured, there is also a debate over the biological influences and implications for increased or decreased T1 and/or ECV that may result with different disease states.
What is the best method for image acquisition?
A central question posed often to the CMR community is which method to use and more specifically which protocol to use. There are numerous approaches described in the literature and that are available to users as research “sequences” [7, 51, 56, 80, 82–85]. However, there are no current standards, and many approaches are not generally accessible. Thus, it is not possible to make a consensus recommendation on a specific method/protocol at this time. There are numerous questions/factors that are important when comparing methods such as: what influences what is really being measured, what is the accuracy and precision of the method, how reproducible is the measurement and how is it affected by variables such as motion, flow, off-resonance, how long is the measurement, and numerous others that are generally relevant in CMR. There are methods based on inversion recovery, saturation recovery, as well as hybrid methods combining inversion and saturation recovery. There are also a number of sampling and fitting strategies, and methods for image reconstruction and motion correction. Furthermore, sampling schemes could be designed differently for pre and post contrast expected T1s for optimal precision.
All of these considerations are important, and in lieu of a consensus protocol at this time, we have recommended more general guidelines for establishing normal baseline values and achieving reproducible measurements. In addition, image acquisition for T1/ECV of thin structures (RV, atria) will require new sophisticated approaches.
Can T1 mapping be performed in all patients?
To achieve wide clinical application, T1 mapping methods should have consistent measurement properties. Any corrections should be properly documented to assure that they can be replicated or reversed if needed. Currently, the properly breath-held single-slice 2D acquisition is preferred. This group could not identify criteria to exclude individuals given the lack of evidence. There are few data examining the degree to which respiratory motion, arrhythmia and the extremes of heart rate, perturb T1 and ECV measures. Furthermore, there are potential disease related factors–thin vs. thick myocardium; presence of arrhythmia; residual heart rate effects that could influence measured T1 and introduce bias to some unknown degree. These topics deserve further investigation
Which level of spatial coverage do we need?
Basal to mid short axis slices are generally preferred given their greater thickness and the generally lower obliquity compared to apical short axis slices. Thin structures and obliquity of myocardium relative to the imaging plane can introduce partial volume effects that inflate native T1 and ECV values [49, 85]. Long axis slices may be more prone to errors related to through plane respiratory motion. The minimum numbers of slices and ideal slice orientation remain undefined. The solution is likely to be different for different diseases (whole heart coverage vs single representative region). These issues deserve further study. In general, for diffuse diseases the mean ECV or T1 seems reasonable to report while for highly regional disease, such as hypertrophic cardiomyopathy, whole heart coverage may eventually become the standard. Peak values may depend on the size of region of interest and the spatial distribution of the disease process being studied.
Impact of GBCA types and concentration on ECV measures?
The concentration of GBCA (or other factors such as cell size) could introduce deviations from the fast exchange limit assumption and influence how much intracellular water is relaxed in any given area of tissue due to trans-cytolemmal water exchange. “Fast water exchange” assumes water exchange is fast between intracellular and extracellular compartments relative to their differences in relaxivity [79, 86]. Higher GBCA concentrations would be more prone to departure from the fast water exchange assumption and potentially measure lower ECV values [78, 86]. Higher concentrations also occur post bolus with early time point measurement, renal impairment and obesity. There is some evidence for this phenomenon with upward drift of ECV measures with longer measurement times post Gd or low bolus doses [56, 63, 72]. Although these effects appear small, further work is needed. There are plenty of potential solutions if required (for example ECV measurement at fixed GBCA concentration; more sophisticated dosing or ECV measurement timing based on lean mass or renal function). However, none of these has been thoroughly investigated at this time.
How should we analyse images?
There are as yet no standard tools to analyse CMR images, with most research being performed using relatively restricted in-house developed tools, early-stage commercial packages, or manufacturer prototypes. Image analysis quality control and quantifying bias from partial volume error is not yet well developed. Automated ECV maps can speed measurement compared to ECV measures from manual regions of interest; additional advantages/disadvantages are unknown. The advantages of two timepoint (pre and post contrast) vs multi-timepoint ECV calculation are also unknown. Multi-timepoint may provide superior robustness to error estimates, but compromise potential spatial coverage and ECV map creation due to additional burden related to processing. Different situations may have different preferred approaches.. The industry is encouraged to provide highly adaptable and robust tools for standardized T1 quantification and map presentation.
What should we report?
The biological significance of the outputs of T1 mapping is not yet known. Multiple parameters can be reported: Global or regional ECV or T1; heterogeneity in areas with or without LGE. Currently, most studies report a singular ECV value per individual, which may be an average over short-axis slice(s) or is sometimes taken from the septum alone. A 16 segment approach however may have problems with regional measurement differences from off resonance effects or partial volume error .
Standardization for clinical utility.
Clinical delivery of T1 mapping at the level of healthcare systems to permit the change of therapy based on T1 measurements has major challenges including magnet QC, normal values and the use of multiple platforms, sequences and contrast agents. In the interim, for multicenter T1 or ECV studies, we recommend performing stratified statistical analyses to adjust for variation related to each site’s scanner characteristics (assuming one scanner per site). Further robust solutions will need to evolve.
Complementary value of multiparametric approaches: native T1, ECV, LGE and others.
Native T1 detects both intracellular and extracellular changes (focal and diffuse). ECV estimates changes in the myocardial interstitium (focal and diffuse). LGE measures focal interstitial changes. There are advantages and disadvantages to each. Native T1 has the advantage of not requiring contrast, detects iron and diffuse fat missed by the other two, but is more sensitive to changes in the type of pulse sequence, including many of its user-dependent parameters, scanner pulse sequence and T1-reconstruction implementation.
Because ECV is a ratio, ECV might be more comparable across platforms and sequences since any systematic biases in T1 estimation may cancel one another, analogous to any volumetric biases incurred during ejection fraction measures. LGE is well established and the gold standard for infarction, provides important diagnostic and prognostic information, but misses diffuse changes [41–47]. Nonetheless, ECV has advantages over LGE for quantifying myocardial fibrosis and the interstitial space. LGE is less suitable for quantifying lesser degrees of ECM expansion [48–54] resulting from pathologies other than myocardial infarction where the differences between normal and affected myocardium are less distinct [20, 49–51, 54, 56, 77, 87, 88]. ECV can detect early fibrosis changes not always detectable by LGE [20, 49–51, 55, 56]. For every disease, the optimal use of these techniques will require exploration. These 3 techniques are also just a subset of tissue characterization techniques with other techniques (T2, diffusion, spectroscopy) and modalities (e.g., ECV by CT)  to be addressed in the future.
Native T1 mapping and ECV may be able to provide important insights into fundamental disease processes affecting the myocardium that otherwise can be difficult to ascertain clinically. Both appear destined to affect clinical decision making but lack multi-centre application and face significant challenges, which demand a community-wide approach (MRI vendors, funding agencies, academics, software companies, contrast agent manufacturers, clinicians). At present, subject to the stated conditions, measures of ECV and native T1 mapping appear sufficiently robust for many diseases; yet more research is required before a large-scale application for clinical decision-making can be recommended. It remains the centre’s responsibility to implement quality control measures, to provide sufficient training for readers and to use validated post-processing and evaluation tools.
James C. Moon acknowledges support from the National Institute for Health Research Biomedical Research Centres funding scheme.
Andrew E. Arai is a principal investigator on a US government Cooperative Research And Development Agreement (CRADA) with Siemens Medical Solutions (HL-CR-05-004).
Matthias G. Friedrich: On the board of directors and shareholder of Circle Cardiovascular Imaging Inc., the manufacturer of a CMR-post processing and evaluation software.
Peter D. Gatehouse. Biomedical Research Unit funding from the National Institute for Health Research. Research agreement with Siemens.
Peter Kellman acknowledges support from the National Heart, Lung and Blood Institute, National Institutes of Health by the Division of Intramural Research, NHLBI, NIH, DHHS (HL004607-14CPB).
Daniel R. Messroghli acknowledges support from the German Research Foundation (DFG) Stefan Neubauer acknowledges support from the National Institute for Health Research Oxford Biomedical Research Centre Programme.
Stefan K. Piechnik acknowledges support from the National Institute for Health Research Oxford Biomedical Research Centre Programme.
SN Matthew D. Robson acknowledges support from the National Institute for Health Research Oxford Biomedical Research Centre Programme.
Erik B. Schelbert acknowledges receipt of contrast material donated by Bracco Diagnostics.
Jeanette Schulz-Menger: Institutional research support from Siemens Healthcare ,Circle Cardiovascular Imaging Inc and Medis. Consultant for Bayer Healthcare.
Martin Ugander acknowledges minor compensation as a speaker for Covidien, and major funding support from the Swedish Research Council. Swedish Heart and Lung Foundation, Stockholm County Council and Karolinska Institutet.
- Moon JC: T1 mapping group minutes. Available at: http://www.scmr.org/forum/viewtopic.php?f=8&t=190&p=1344#p1344. Accessed June 22, 2013
- Messroghli DR, Niendorf T, Schulz-Menger J, Dietz R, Friedrich MG: T1 mapping in patients with acute myocardial infarction. J. Cardiovasc. Magn. Reson. 2003, 5: 353-59.View ArticlePubMedGoogle Scholar
- Messroghli DR, Radjenovic A, Kozerke S, Higgins DM, Sivananthan MU, Ridgway JP: Modified look-locker inversion recovery (MOLLI) for high-resolution T1 mapping of the heart. Magn. Reson. Med. 2004, 52: 141-46.View ArticlePubMedGoogle Scholar
- Brading AF, Jones AW: Distribution and kinetics of CoEDTA in smooth muscle, and its use as an extracellular marker. J. Physiol. 1969, 200: 387-401.PubMed CentralView ArticlePubMedGoogle Scholar
- Poole-Wilson PA: The intracellular pH, potassium and electrolyte content of heart muscle in acidosis and alkalosis. 1975, London: University of CambridgeGoogle Scholar
- Arheden H, Saeed M, Higgins CB, Gao DW, Bremerich J, Wyttenbach R, Dae MW, Wendland MF: Measurement of the distribution volume of gadopentetate dimeglumine at echo-planar MR imaging to quantify myocardial infarction: comparison with 99mTc-DTPA autoradiography in rats. Radiology. 1999, 211: 698-708.View ArticlePubMedGoogle Scholar
- Jerosch-Herold M, Sheridan DC, Kushner JD, Nauman D, Burgess D, Dutton D, Alharethi R, Li D, Hershberger RE: Cardiac magnetic resonance imaging of myocardial contrast uptake and blood flow in patients affected with idiopathic or familial dilated cardiomyopathy. Am. J. Physiol. Heart Circ. Physiol. 2008, 295: H1234-42.PubMed CentralView ArticlePubMedGoogle Scholar
- Ugander M, Bagi PS, Oki AJ, Chen B, Hsu LY, Aletras AH, Shah S, Greiser A, Kellman P, Arai AE: Myocardial edema as detected by pre-contrast T1 and T2 CMR delineates area at risk associated with acute myocardial infarction. JACC Cardiovasc. Imaging. 2012, 5: 596-603.PubMed CentralView ArticlePubMedGoogle Scholar
- Ferreira VM, Piechnik SK, Dall’Armellina E, Karamitsos TD, Francis JM, Choudhury RP, Friedrich MG, Robson MD, Neubauer S: Non-contrast T1-mapping detects acute myocardial edema with high diagnostic accuracy: a comparison to T2-weighted cardiovascular magnetic resonance. J. Cardiovasc. Magn. Reson. 2012, 14: 42-PubMed CentralView ArticlePubMedGoogle Scholar
- Karamitsos TD, Piechnik SK, Banypersad SM, Fontana M, Ntusi NB, Ferreira VM, Whelan CJ, Myerson SG, Robson MD, Hawkins PN, Neubauer S, Moon JC: Noncontrast T1 mapping for the diagnosis of cardiac amyloidosis. JACC Cardiovasc. Imaging. 2013, 6: 488-97.View ArticlePubMedGoogle Scholar
- Bull S, White SK, Piechnik SK, Flett AS, Ferreira VM, Loudon M, Francis JM, Karamitsos TD, Prendergast BD, Robson MD, Neubauer S, Moon JC, Myerson SG: Human non-contrast T1 values and correlation with histology in diffuse fibrosis. Heart. 2013, 99: 932-37.PubMed CentralView ArticlePubMedGoogle Scholar
- Sado DM, White SK, Piechnik SK, Banypersad SM, Treibel T, Captur G, Fontana M, Maestrini V, Flett AS, Robson MD, Lachmann RH, Murphy E, Mehta A, Hughes D, Neubauer S, Elliott PM, Moon JC: Identification and assessment of anderson-fabry disease by cardiovascular magnetic resonance noncontrast myocardial t1 mapping. Circ. Cardiovasc. Imaging. 2013, 6: 392-98.View ArticlePubMedGoogle Scholar
- Scholz TD, Fleagle SR, Parrish FC, Breon T, Skorton DJ: Effect of tissue fat and water content on nuclear magnetic resonance relaxation times of cardiac and skeletal muscle. Magn. Reson. Imaging. 1990, 8: 605-11.View ArticlePubMedGoogle Scholar
- Pedersen SF, Thrysoe SA, Robich MP, Paaske WP, Ringgaard S, Botker HE, Hansen ES, Kim WY: Assessment of intramyocardial hemorrhage by T1-weighted cardiovascular magnetic resonance in reperfused acute myocardial infarction. J. Cardiovasc. Magn. Reson. 2012, 14: 59-PubMed CentralView ArticlePubMedGoogle Scholar
- Puntmann VO, D’Cruz D, Smith Z, Pastor A, Choong P, Voigt T, Carr-White G, Sangle S, Schaeffter T, Nagel E: Native myocardial T1 mapping by cardiovascular magnetic resonance imaging in subclinical cardiomyopathy in patients with systemic lupus erythematosus. Circ. Cardiovasc. Imaging. 2013, 6: 295-301.View ArticlePubMedGoogle Scholar
- Sado DM, White SK, Piechnik SK, Banypersad SM, Treibel T, Captur G, Fontana M, Maestrini V, Flett AS, Robson MD, Lachmann RH, Murphy E, Mehta A, Hughes D, Neubauer S, Elliott PM, Moon JC: The identification and assessment of Anderson Fabry disease by cardiovascular magnetic resonance non-contrast myocardial T1 mapping. Circ. Cardiovasc. Imaging. 2013, 6: 392-8.View ArticlePubMedGoogle Scholar
- Dall’Armellina E, Piechnik SK, Ferreira VM, Si QL, Robson MD, Francis JM, Cuculi F, Kharbanda RK, Banning AP, Choudhury RP, Karamitsos TD, Neubauer S: Cardiovascular magnetic resonance by non contrast T1-mapping allows assessment of severity of injury in acute myocardial infarction. J. Cardiovasc. Magn. Reson. 2012, 14: 15-PubMed CentralView ArticlePubMedGoogle Scholar
- Ferreira VM, Piechnik SK, Dall’armellina E, Karamitsos TD, Francis JM, Ntusi N, Holloway C, Choudhury RP, Kardos A, Robson MD, Friedrich MG, Neubauer S: T mapping for the diagnosis of acute myocarditis using CMR: comparison to T-weighted and late gadolinium enhanced imaging. JACC Cardiovasc Imaging. 2013, doi:10.1016/j.jcmg.2013.03.008 [Epud ahead of print]Google Scholar
- Weber KT, Brilla CG: Pathological hypertrophy and cardiac interstitium: fibrosis and renin-angiotensin-aldosterone system. Circulation. 1991, 83: 1849-65.View ArticlePubMedGoogle Scholar
- Wong TC, Piehler K, Meier CG, Testa SM, Klock AM, Aneizi AA, Shakesprere J, Kellman P, Shroff SG, Schwartzman DS, Mulukutla SR, Simon MA, Schelbert EB: Association between extracellular matrix expansion quantified by cardiovascular magnetic resonance and short-term mortality. Circulation. 2012, 126: 1206-16.PubMed CentralView ArticlePubMedGoogle Scholar
- Wong TC, Piehler K, Kang IA, Kadakkal A, Kellman P, Schwartzman DS, Mulukutla SR, Simon MA, Shroff SG, Kuller LH, Schelbert EB: Myocardial extracellular volume fraction quantified by cardiovascular magnetic resonance is increased in diabetes and associated with mortality and incident heart failure admission. Eur. Heart J. 2013, doi: 10.1093/eurheartj/eht193Google Scholar
- Butler J, Fonarow GC, Gheorghiade M: Strategies and opportunities for drug development in heart failure. JAMA. 2013, 309: 1593-94.View ArticlePubMedGoogle Scholar
- Banypersad SM, Sado DM, Flett AS, Gibbs SDJ, Pinney JH, Maestrini V, Cox AT, Fontana M, Whelan CJ, Wechalekar AD, Hawkins PN, Moon JC: Quantification of myocardial extracellular volume fraction in systemic AL amyloidosis: an equilibrium contrast cardiovascular magnetic resonance study. Circ. Cardiovasc. Imaging. 2012, 6: 34-9.View ArticlePubMedGoogle Scholar
- Fontana M, White SK, Banypersad SM, Sado DM, Maestrini V, Flett AS, Piechnik SK, Neubauer S, Roberts N, Moon JC: Comparison of T1 mapping techniques for ECV quantification: histological validation and reproducibility of ShMOLLI versus multibreath-hold T1 quantification equilibrium contrast CMR. J. Cardiovasc. Magn. Reson. 2012, 14: 88-PubMed CentralView ArticlePubMedGoogle Scholar
- Brilla CG, Funck RC, Rupp H: Lisinopril-mediated regression of myocardial fibrosis in patients with hypertensive heart disease. Circulation. 2000, 102: 1388-93.View ArticlePubMedGoogle Scholar
- Diez J, Querejeta R, Lopez B, Gonzalez A, Larman M, Martinez Ubago JL: Losartan-dependent regression of myocardial fibrosis is associated with reduction of left ventricular chamber stiffness in hypertensive patients. Circulation. 2002, 105: 2512-17.View ArticlePubMedGoogle Scholar
- Izawa H, Murohara T, Nagata K, Isobe S, Asano H, Amano T, Ichihara S, Kato T, Ohshima S, Murase Y, Iino S, Obata K, Noda A, Okumura K, Yokota M: Mineralocorticoid receptor antagonism ameliorates left ventricular diastolic dysfunction and myocardial fibrosis in mildly symptomatic patients with idiopathic dilated cardiomyopathy: a pilot study. Circulation. 2005, 112: 2940-45.PubMedGoogle Scholar
- McLenachan JM, Dargie HJ: Ventricular arrhythmias in hypertensive left ventricular hypertrophy: relationship to coronary artery disease, left ventricular dysfunction, and myocardial fibrosis. Am. J. Hypertens. 1990, 3: 735-40.PubMedGoogle Scholar
- Kawara T, Derksen R, de Groot JR, Coronel R, Tasseron S, Linnenbank AC, Hauer RN, Kirkels H, Janse MJ, De Bakker JM: Activation delay after premature stimulation in chronically diseased human myocardium relates to the architecture of interstitial fibrosis. Circulation. 2001, 104: 3069-75.View ArticlePubMedGoogle Scholar
- Anderson KP, Walker R, Urie P, Ershler PR, Lux RL, Karwandee SV: Myocardial electrical propagation in patients with idiopathic dilated cardiomyopathy. J. Clin. Invest. 1993, 92: 122-40.PubMed CentralView ArticlePubMedGoogle Scholar
- Tamarappoo BK, John BT, Reinier K, Teodorescu C, Uy-Evanado A, Gunson K, Jui J, Chugh SS: Vulnerable myocardial interstitium in patients with isolated left ventricular hypertrophy and sudden cardiac death: a postmortem histological evaluation. J Am Heart Assoc. 2012, 1: e001511-PubMed CentralView ArticlePubMedGoogle Scholar
- Schwartzkopff B, Brehm M, Mundhenke M, Strauer BE: Repair of coronary arterioles after treatment with perindopril in hypertensive heart disease. Hypertension. 2000, 36: 220-25.View ArticlePubMedGoogle Scholar
- Naghavi M, Libby P, Falk E, Casscells SW, Litovsky S, Rumberger J, Badimon JJ, Stefanadis C, Moreno P, Pasterkamp G, Fayad Z, Stone PH, Waxman S, Raggi P, Madjid M, Zarrabi A, Burke A, Yuan C, Fitzgerald PJ, Siscovick DS, De Korte CL, Aikawa M, Juhani Airaksinen KE, Assmann G, Becker CR, Chesebro JH, Farb A, Galis ZS, Jackson C, Jang IK, et al: From vulnerable plaque to vulnerable patient: a call for new definitions and risk assessment strategies: Part I. Circulation. 2003, 108: 1664-72.View ArticlePubMedGoogle Scholar
- Khavandi K, Khavandi A, Asghar O, Greenstein A, Withers S, Heagerty AM, Malik RA: Diabetic cardiomyopathy–a distinct disease?. Best Pract. Res. Clin. Endocrinol. Metab. 2009, 23: 347-60.View ArticlePubMedGoogle Scholar
- Asbun J, Villarreal FJ: The pathogenesis of myocardial fibrosis in the setting of diabetic cardiomyopathy. J. Am. Coll. Cardiol. 2006, 47: 693-700.View ArticlePubMedGoogle Scholar
- Goser S, Andrassy M, Buss SJ, Leuschner F, Volz CH, Ottl R, Zittrich S, Blaudeck N, Hardt SE, Pfitzer G, Rose NR, Katus HA, Kaya Z: Cardiac troponin I but not cardiac troponin T induces severe autoimmune inflammation in the myocardium. Circulation. 2006, 114: 1693-702.View ArticlePubMedGoogle Scholar
- Van Hoeven KH, Factor SM: A comparison of the pathological spectrum of hypertensive, diabetic, and hypertensive-diabetic heart disease. Circulation. 1990, 82: 848-55.View ArticlePubMedGoogle Scholar
- Wynn TA, Ramalingam TR: Mechanisms of fibrosis: therapeutic translation for fibrotic disease. Nat. Med. 2012, 18: 1028-40.PubMed CentralView ArticlePubMedGoogle Scholar
- Beltrami CA, Finato N, Rocco M, Feruglio GA, Puricelli C, Cigola E, Quaini F, Sonnenblick EH, Olivetti G, Anversa P: Structural basis of end-stage failure in ischemic cardiomyopathy in humans. Circulation. 1994, 89: 151-63.View ArticlePubMedGoogle Scholar
- Beltrami CA, Finato N, Rocco M, Feruglio GA, Puricelli C, Cigola E, Sonnenblick EH, Olivetti G, Anversa P: The cellular basis of dilated cardiomyopathy in humans. J. Mol. Cell. Cardiol. 1995, 27: 291-305.View ArticlePubMedGoogle Scholar
- Schelbert EB, Cao JJ, Sigurdsson S, Aspelund T, Kellman P, Aletras AH, Dyke CK, Thorgeirsson G, Eiriksdottir G, Launer LJ, Gudnason V, Harris TB, Arai AE: Prevalence and prognosis of unrecognized myocardial infarction determined by cardiac magnetic resonance in older adults. JAMA. 2012, 308: 890-96.PubMed CentralView ArticlePubMedGoogle Scholar
- Wong TC, Piehler K, Puntil KS, Moguillansky D, Meier CG, Lacomis JL, Kellman P, Cook SC, Schwartzman DS, Simon MA, Mulukutla SR, Schelbert EB: Effectiveness of late gadolinium enhancement to improve outcomes prediction in patients referred for cardiovascular magnetic resonance after echocardiography. J. Cardiovasc. Magn. Reson. 2013, 15: 1-View ArticleGoogle Scholar
- Cheong BY, Muthupillai R, Wilson JM, Sung A, Huber S, Amin S, Elayda MA, Lee VV, Flamm SD: Prognostic significance of delayed-enhancement magnetic resonance imaging: survival of 857 patients with and without left ventricular dysfunction. Circulation. 2009, 120: 2069-76.View ArticlePubMedGoogle Scholar
- Klem I, Shah DJ, White RD, Pennell DJ, Van Rossum AC, Regenfus M, Sechtem U, Schvartzman PR, Hunold P, Croisille P, Parker M, Judd RM, Kim RJ: Prognostic value of routine cardiac magnetic resonance assessment of left ventricular ejection fraction and myocardial damage: an international multicenter study. Circ. Cardiovasc. Imaging. 2011, 4: 610-19.View ArticlePubMedGoogle Scholar
- Kim RJ, Wu E, Rafael A, Chen EL, Parker MA, Simonetti O, Klocke FJ, Bonow RO, Judd RM: The use of contrast-enhanced magnetic resonance imaging to identify reversible myocardial dysfunction. N. Engl. J. Med. 2000, 343: 1445-53.View ArticlePubMedGoogle Scholar
- Gulati A, Jabbour A, Ismail TF, Guha K, Khwaja J, Raza S, Morarji K, Brown TD, Ismail NA, Dweck MR, Di Pietro E, Roughton M, Wage R, Daryani Y, O’Hanlon R, Sheppard MN, Alpendurada F, Lyon AR, Cook SA, Cowie MR, Assomull RG, Pennell DJ, Prasad SK: Association of fibrosis with mortality and sudden cardiac death in patients with nonischemic dilated cardiomyopathy. JAMA. 2013, 309: 896-908.View ArticlePubMedGoogle Scholar
- De Waha S, Desch S, Eitel I, Fuernau G, Zachrau J, Leuschner A, Gutberlet M, Schuler G, Thiele H: Impact of early vs. late microvascular obstruction assessed by magnetic resonance imaging on long-term outcome after ST-elevation myocardial infarction: a comparison with traditional prognostic markers. Eur. Heart J. 2010, 31: 2660-68.View ArticlePubMedGoogle Scholar
- Sado DM, Flett AS, Banypersad SM, White SK, Maestrini V, Quarta G, Lachmann RH, Murphy E, Mehta A, Hughes DA, McKenna WJ, Taylor AM, Hausenloy DJ, Hawkins PN, Elliott PM, Moon JC: Cardiovascular magnetic resonance measurement of myocardial extracellular volume in health and disease. Heart. 2012, 98: 1436-41.View ArticlePubMedGoogle Scholar
- Kellman P, Wilson JR, Xue H, Bandettini WP, Shanbhag SM, Druey KM, Ugander M, Arai AE: Extracellular volume fraction mapping in the myocardium, Part 2: initial clinical experience. J. Cardiovasc. Magn. Reson. 2012, 14: 64-PubMed CentralView ArticlePubMedGoogle Scholar
- Broberg CS, Chugh SS, Conklin C, Sahn DJ, Jerosch-Herold M: Quantification of diffuse myocardial fibrosis and its association with myocardial dysfunction in congenital heart disease. Circ. Cardiovasc. Imaging. 2010, 3: 727-34.PubMed CentralView ArticlePubMedGoogle Scholar
- Ugander M, Oki AJ, Hsu LY, Kellman P, Greiser A, Aletras AH, Sibley CT, Chen MY, Bandettini WP, Arai AE: Extracellular volume imaging by magnetic resonance imaging provides insights into overt and sub-clinical myocardial pathology. Eur. Heart J. 2012, 33: 1268-78.PubMed CentralView ArticlePubMedGoogle Scholar
- Flett AS, Sado DM, Quarta G, Mirabel M, Pellerin D, Herrey AS, Hausenloy DJ, Ariti C, Yap J, Kolvekar S, Taylor AM, Moon JC: Diffuse myocardial fibrosis in severe aortic stenosis: an equilibrium contrast cardiovascular magnetic resonance study. Eur Heart J Cardiovasc Imaging. 2012, 13: 819-26.View ArticlePubMedGoogle Scholar
- Mongeon FP, Jerosch-Herold M, Coelho-Filho OR, Blankstein R, Falk RH, Kwong RY: Quantification of extracellular matrix expansion by CMR in infiltrative heart disease. JACC Cardiovasc. Imaging. 2012, 5: 897-907.PubMed CentralView ArticlePubMedGoogle Scholar
- Flett AS, Hayward MP, Ashworth MT, Hansen MS, Taylor AM, Elliott PM, McGregor C, Moon JC: Equilibrium contrast cardiovascular magnetic resonance for the measurement of diffuse myocardial fibrosis: preliminary validation in humans. Circulation. 2010, 122: 138-44.View ArticlePubMedGoogle Scholar
- Schalla S, Bekkers SC, Dennert R, Van Suylen RJ, Waltenberger J, Leiner T, Wildberger J, Crijns HJ, Heymans S: Replacement and reactive myocardial fibrosis in idiopathic dilated cardiomyopathy: comparison of magnetic resonance imaging with right ventricular biopsy. Eur. J. Heart Fail. 2010, 12: 227-31.View ArticlePubMedGoogle Scholar
- Schelbert EB, Testa SM, Meier CG, Ceyrolles WJ, Levenson JE, Blair AJ, Kellman P, Jones BL, Ludwig DR, Schwartzman D, Shroff SG, Wong TC: Myocardial extravascular extracellular volume fraction measurement by gadolinium cardiovascular magnetic resonance in humans: slow infusion versus bolus. J. Cardiovasc. Magn. Reson. 2011, 13: 16-PubMed CentralView ArticlePubMedGoogle Scholar
- Shah RV, Abbasi SA, Neilan TG, Hulten E, Coelho-Filho O, Hoppin A, Levitsky L, De Ferranti S, Rhodes ET, Traum A, Goodman E, Feng H, Heydari B, Harris WS, Hoefner DM, McConnell JP, Seethamraju R, Rickers C, Kwong RY, Jerosch-Herold M: Myocardial tissue remodeling in adolescent obesity. J Am Heart Assoc. 2013, 2: e000279-PubMed CentralView ArticlePubMedGoogle Scholar
- Tham EB, Haykowsky MJ, Chow K, Spavor M, Kaneko S, Khoo NS, Pagano JJ, Mackie AS, Thompson RB: Diffuse myocardial fibrosis by T1-mapping in children with subclinical anthracycline cardiotoxicity: relationship to exercise capacity, cumulative dose and remodeling. J. Cardiovasc. Magn. Reson. 2013, 15: 48-PubMed CentralView ArticlePubMedGoogle Scholar
- Kellman P, Wilson JR, Xue H, Ugander M, Arai AE: Extracellular volume fraction mapping in the myocardium, Part 1: evaluation of an automated method. J. Cardiovasc. Magn. Reson. 2012, 14: 63-PubMed CentralView ArticlePubMedGoogle Scholar
- Tyralla K, Adamczak M, Benz K, Campean V, Gross ML, Hilgers KF, Ritz E, Amann K: High-dose enalapril treatment reverses myocardial fibrosis in experimental uremic cardiomyopathy. PLoS One. 2011, 6: e15287-PubMed CentralView ArticlePubMedGoogle Scholar
- Roffe C: Ageing of the heart. Br. J. Biomed. Sci. 1998, 55: 136-48.PubMedGoogle Scholar
- Urbieta-Caceres VH, Zhu XY, Gibson ME, Favreau FD, Jordan K, Lerman A, Lerman LO: Reversal of experimental renovascular hypertension restores coronary microvascular function and architecture. Am. J. Hypertens. 2011, 24: 458-65.PubMed CentralView ArticlePubMedGoogle Scholar
- Miller CA, Naish J, Bishop P, Coutts G, Clark D, Zhao S, Ray SG, Yonan N, Williams SG, Flett AS, Moon JC, Greiser A, Parker GJ, Schmitt M: Comprehensive validation of cardiovascular magnetic resonance techniques for the assessment of myocardial extracellular volume. Circ Cardiovasc Imaging. 2013, 6: 373-83.View ArticlePubMedGoogle Scholar
- Anderson KR, Sutton MG, Lie JT: Histopathological types of cardiac fibrosis in myocardial disease. J. Pathol. 1979, 128: 79-85.View ArticlePubMedGoogle Scholar
- Swynghedauw B: Molecular mechanisms of myocardial remodeling. Physiol. Rev. 1999, 79: 215-62.PubMedGoogle Scholar
- White SK, Sado DM, Fontana M, Banypersad SM, Maestrini V, Flett AS, Piechnik SK, Robson MD, Hausenloy DJ, Sheikh AM, Hawkins PN, Moon JC: T1 mapping for myocardial extracellular volume measurement by CMR: bolus only versus primed infusion technique. JACC Cardiovasc. Imaging. 2013, doi: 10.1016/j.jcmg.2013.01.011Google Scholar
- Kellman P, Arai AE, Xue H: T1 and extracellular volume mapping in the heart: estimation of error maps and the influence of noise on precision. J. Cardiovasc. Magn. Reson. 2013, 15: 56-PubMed CentralView ArticlePubMedGoogle Scholar
- Bossuyt PM, Reitsma JB, Bruns DE, Gatsonis CA, Glasziou PP, Irwig LM, Lijmer JG, Moher D, Rennie D, De Vet HC: Towards complete and accurate reporting of studies of diagnostic accuracy: the STARD initiative. BMJ. 2003, 326: 41-4.PubMed CentralView ArticlePubMedGoogle Scholar
- Robson MD, Piechnik SK, Tunnicliffe EM, Neubauer S: T1 measurements in the human myocardium: the effects of magnetization transfer on the SASHA and MOLLI sequences. Magn Reson Med. 2013, doi:10.1002/mrm.24867 [Epub ahead of print]Google Scholar
- Iles L, Pfluger H, Phrommintikul A, Cherayath J, Aksit P, Gupta SN, Kaye DM, Taylor AJ: Evaluation of diffuse myocardial fibrosis in heart failure with cardiac magnetic resonance contrast-enhanced T1 mapping. J. Am. Coll. Cardiol. 2008, 52: 1574-80.View ArticlePubMedGoogle Scholar
- Sibley CT, Noureldin RA, Gai N, Nacif MS, Liu S, Turkbey EB, Mudd JO, van der Geest RJ, Lima JA, Halushka MK, Bluemke DA: T1 mapping in cardiomyopathy at cardiac MR: comparison with endomyocardial biopsy. Radiology. 2012, 265: 724-32.PubMed CentralView ArticlePubMedGoogle Scholar
- Kawel N, Nacif M, Zavodni A, Jones J, Liu S, Sibley CT, Bluemke DA: T1 mapping of the myocardium: intra-individual assessment of post-contrast T1 time evolution and extracellular volume fraction at 3T for Gd-DTPA and Gd-BOPTA. J. Cardiovasc. Magn. Reson. 2012, 14: 26-PubMed CentralView ArticlePubMedGoogle Scholar
- Markl M, Pelc NJ: On flow effects in balanced steady-state free precession imaging: pictorial description, parameter dependence, and clinical implications. J. Magn. Reson. Imaging. 2004, 20: 697-705.View ArticlePubMedGoogle Scholar
- Kellman P, Herzka DA, Arai AE, Hansen MS: Influence of off-resonance in myocardial T1-mapping using SSFP based MOLLI method. J. Cardiovasc. Magn. Reson. 2013, 15: 63-PubMed CentralView ArticlePubMedGoogle Scholar
- Hunold P, Schlosser T, Vogt FM, Eggebrecht H, Schmermund A, Bruder O, Schuler WO, Barkhausen J: Myocardial late enhancement in contrast-enhanced cardiac MRI: distinction between infarction scar and non-infarction-related disease. AJR Am. J. Roentgenol. 2005, 184: 1420-26.View ArticlePubMedGoogle Scholar
- Mahrholdt H, Wagner A, Judd RM, Sechtem U, Kim RJ: Delayed enhancement cardiovascular magnetic resonance assessment of non-ischaemic cardiomyopathies. Eur. Heart J. 2005, 26: 1461-74.View ArticlePubMedGoogle Scholar
- Flett AS, Hasleton J, Cook C, Hausenloy D, Quarta G, Ariti C, Muthurangu V, Moon JC: Evaluation of techniques for the quantification of myocardial scar of differing etiology using cardiac magnetic resonance. JACC Cardiovasc. Imaging. 2011, 4: 150-56.View ArticlePubMedGoogle Scholar
- Zhou R, Pickup S, Yankeelov TE, Springer CS, Glickson JD: Simultaneous measurement of arterial input function and tumor pharmacokinetics in mice by dynamic contrast enhanced imaging: effects of transcytolemmal water exchange. Magn. Reson. Med. 2004, 52: 248-57.View ArticlePubMedGoogle Scholar
- Landis CS, Li X, Telang FW, Molina PE, Palyka I, Vetek G, Springer CS: Equilibrium transcytolemmal water-exchange kinetics in skeletal muscle in vivo. Magn. Reson. Med. 1999, 42: 467-78.View ArticlePubMedGoogle Scholar
- Coelho-Filho OR, Mongeon FP, Mitchell R, Moreno H, Nadruz W, Kwong R, Jerosch-Herold M: The role of transcytolemmal water exchange in magnetic resonance measurements of diffuse myocardial fibrosis in hypertensive heart disease. Circ Cardiovasc Imaging. 2013, 134: 41-Google Scholar
- Messroghli DR, Plein S, Higgins DM, Walters K, Jones TR, Ridgway JP, Sivananthan MU: Human myocardium: single-breath-hold MR T1 mapping with high spatial resolution–reproducibility study. Radiology. 2006, 238: 1004-12.View ArticlePubMedGoogle Scholar
- Messroghli DR, Greiser A, Frohlich M, Dietz R, Schulz-Menger J: Optimization and validation of a fully-integrated pulse sequence for modified look-locker inversion-recovery (MOLLI) T1 mapping of the heart. J. Magn. Reson. Imaging. 2007, 26: 1081-86.View ArticlePubMedGoogle Scholar
- Piechnik SK, Ferreira VM, Dall’Armellina E, Cochlin LE, Greiser A, Neubauer S, Robson MD: Shortened modified look-locker inversion recovery (ShMOLLI) for clinical myocardial T1-mapping at 1.5 and 3 T within a 9 heartbeat breathhold. J. Cardiovasc. Magn. Reson. 2010, 12: 69-PubMed CentralView ArticlePubMedGoogle Scholar
- Chow K, Flewitt JA, Green JD, Pagano JJ, Friedrich MG, Thompson RB: Saturation recovery single-shot acquisition (SASHA) for myocardial T mapping. Magn Reson Med. 2013, doi:10.1002/mrm.24878 [Epud ahead of print]Google Scholar
- Piechnik SK, Ferreira VM, Lewandowski AJ, Ntusi NA, Banerjee R, Holloway C, Hofman MB, Sado DM, Maestrini V, White SK, Lazdam M, Karamitsos T, Moon JC, Neubauer S, Leeson P, Robson MD: Normal variation of magnetic resonance T1 relaxation times in the human population at 1.5 T using ShMOLLI. J. Cardiovasc. Magn. Reson. 2013, 15: 13-PubMed CentralView ArticlePubMedGoogle Scholar
- Coelho-Filho OR, Mongeon FP, Mitchell R, Moreno H, Nadruz W, Kwong R, Jerosch-Herold M: Role of transcytolemmal water-exchange in magnetic resonance measurements of diffuse myocardial fibrosis in hypertensive heart disease. Circ. Cardiovasc. Imaging. 2013, 6: 134-41.PubMed CentralView ArticlePubMedGoogle Scholar
- Sado DM, Flett AS, Moon JC: Novel imaging techniques for diffuse myocardial fibrosis. Future Cardiol. 2011, 7: 643-50.View ArticlePubMedGoogle Scholar
- Kwong RY, Farzaneh-Far A: Measuring myocardial scar by CMR. JACC Cardiovasc. Imaging. 2011, 4: 157-60.View ArticlePubMedGoogle Scholar
- Nacif MS, Kawel N, Lee JJ, Chen X, Yao J, Zavodni A, Sibley CT, Lima JA, Liu S, Bluemke DA: Interstitial myocardial fibrosis assessed as extracellular volume fraction with low-radiation-dose cardiac CT. Radiology. 2012, 264: 876-83.PubMed CentralView ArticlePubMedGoogle Scholar
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