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- Open Access
CT and quantitative 19F MRI tracking of encapsulated mesenchymal stem cells in a peripheral arterial disease rabbit model
© Wang et al; licensee BioMed Central Ltd. 2013
- Published: 30 January 2013
- Peripheral Arterial Disease
- Fluorine Concentration
- Microencapsuled Cell
- Conventional Angioplasty
- Microencapsulation Method
Nearly 12% of Americans suffer from peripheral arterial disease (PAD) and many are not eligible for conventional angioplasty or surgical bypass. Microencapsulated stem cell (SC) therapy offers a novel means to transplant allogeneic SCs to avoid immunorejection and enable image tracking. However, quantitative evaluation of capsule fate is elusive. Here we explore c-arm CT and 19F-MRI for serial evaluation of dual X-ray/MR-visible SC microcapsules (XMRCaps) in a rabbit PAD model.
XMRCaps were produced using a modified alginate microencapsulation method impregnating perfluorooctylbromine (PFOB) and human or rabbit SCs. In vitro validation studies were performed in a phantom consisting of known quantities of XMRCaps. C-arm CT images (dynaCT, Siemens Artis Zee) were acquired and reconstructed at 0.46 mm3 voxel size. 19F MRI was acquired with a flexible, 4-channel Tx/Rx 19F coil (3D TrueFISP, Siemens Tim Trio, 4.1 ms TR; 2.0 ms TE; 32 averages) in the coronal plane. 1H MRI was acquired with the system body or body matrix coil.
For in vivo studies, c-arm CTs and MRIs were acquired at one, two, and three weeks after human SC (XenoSC) or rabbit SC (AlloSC) XMRCap intramuscular administration in the hindlimb of a PAD rabbit (n=4) using identical imaging parameters as the in vitro studies. To test the repeatability of 19F MRI, imaging sets were acquired twice on the same day in one rabbit with the 19F coil repositioned in between. To enhance repeatability, the fluorine coil was constrained to a fixed geometry. Reference PFOB markers were placed within the imaging field to enable quantitation of XMRCaps.
Segmentation of the injections sites in c-arm CT and 19F MRI was performed with a thresholding algorithm. Relative fluorine concentration was determined by averaging the integrated 19F signal intensity over the segmented volume after normalization to the 19F standards.
MRI provides accurate assessment of XMRCap volumes with increased MRI volumes due to partial volume effects of the larger MRI voxel. In vivo volume changes of XMRCaps injection sites in the ischemic environment of the PAD rabbit could be quantified on MRI and CT. However, only MRI was able to demonstrate alterations in the fluorine concentration. In particular, decreases in XMRCap volumes with stable or increasing fluorine concentrations in animals that received allogeneic stem cells suggest better tolerance of allogenic microencapsuled cells than xenogenic stem cells.
a grant from Siemens AG, NIH R33-HL089029, and the Maryland Stem Cell Research Foundation (2008-MDSCRFII-0399).
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.