- Oral presentation
- Open Access
Longitudinal monitoring of cardiac dysfunction with MRI in a mouse model of obesity and type 2 diabetes
© Abdesselam et al.; licensee BioMed Central Ltd. 2014
- Published: 16 January 2014
- Insulin Resistance
- Arterial Spin Label
- Cardiac Perfusion
- Sucrose Diet
- High Sucrose Diet
Obesity consequences include type 2 diabetes and cardiovascular diseases. Ectopic fat deposition within certain organs such as heart and liver, have recently been shown to be important independent risk factors for the development of these diseases. The purpose of this study was to perform a longitudinal analysis of the changes in cardio-metabolic functions associated with adiposity increase and insulin resistance development.
Male C57BL6R mice were subjected to a high fat high sucrose diet (HFHSD, N = 10) or a regular show diet (RD, N = 10) during 4 months in order to induce obesity and insulin resistance. MRI was performed on a Bruker Biospec AVANCE 4.7T system for animal examination. Mice underwent complete exploration every month as follows: 1/determination of body fat content and distribution including ectopic fat using MRS with PRESS sequence and body imaging, 2/cardiac function assessment with 4-cavity and short-axis cine sequences, and 3/cardiac perfusion investigation with arterial spin labeling .
These preliminary data established the chronological order of anomalies appearance associated to diet-induced obesity and insulin resistance in a 4 month longitudinal assessment. Hepatic triglyceride content seems to be a relevant predictive factor of cardiac anomalies. This experimental protocol might be useful to assess the effect of anti-diabetic drugs on cardiac function.
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