- Oral presentation
- Open Access
Manganese-enhanced MRI enables longitudinal tracking of transplanted stem cell viability in the murine myocardium
© Dash et al.; licensee BioMed Central Ltd. 2014
- Published: 16 January 2014
- Stem Cell
- Mesenchymal Stem Cell
- Stem Cell Therapy
- Human Placenta
- Luciferase Reporter Gene
Stem cell therapy in the heart is limited by an inability to track transplanted cell survival. To address this limitation, we used human amnion-derived mesenchymal stem cells (hAMSCs), which exhibit longer in vivo survival, and Manganese (Mn2+)-Enhanced MRI (MEMRI), which enters live stem cells to augment T1 signal. We tested Mn2+ pre-labeling of hAMSCs in vitro and whether MEMRI would detect hAMSC survival in mouse myocardium in vivo.
hAMSCs were isolated from human placentas after IRB consent. A subset of cells was transduced with a luciferase reporter gene. One group of hAMSCs was exposed to 1 μM doxorubicin (DOX) for 4 hrs, then incubated for 48 hrs. The hAMSCs (Healthy & DOX) were then labeled with increasing concentrations (0.1, 0.5, & 1 mM) of MnCl2 (Sigma, Inc) for 30 min. Bioluminescence (BLI) was performed after MnCl2 labeling. Cells were pelleted into Eppendorf tubes and in vitro 3T MRI was performed (SignaHDx, GE, Inc). For in vivo MEMRI, 0.25 × 106 Healthy and DOX hAMSCs were pre-labeled with 0.5 mM MnCl2 for 30 min, washed, and pelleted for direct injection into hindlimb & myocardium. Mice were immediately imaged using an FGRE-irP sequence: FOV4/ST 1 mm/TE min/TI 400 ms/NEX4. In vivo MEMRI was repeated 2 days later, after 250 μl of i.p. MnCl2.
MEMRI successfully labels and tracks live, transplanted hAMSCs in the heart, enabling serial tracking of cell delivery and survival with no genetic pre-modification.
NIH K08 (RD) NIH R01 (PY) Stanford CVI Seed Grant (RD).
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.