Ex vivo cardiac DTI: on the effects of diffusion time and b-value
© Froeling et al.; licensee BioMed Central Ltd. 2014
Published: 16 January 2014
For in-vivo an ex-vivo studies of myocardial structure, generally a spin echo (SE) or stimulated echo (STE) based sequence is used. Diffusion weighting is reported with a large range of diffusion times (50 and 1000 ms). Furthermore, in-vivo studies generally use low b-values (300 s/mm2) whereas ex-vivo experiments allow for much higher b-values. For interpretation of in-vivo cardiac DTI a understanding of the effects of sequence parameters on the derived diffusion parameters is needed. In this study, we therefore studied the impact of diffusion time and b-value on these parameters.
Experiments were performed on ex-vivo pig hearts stored in 4% paraformaldehyde for at least three weeks. 24 hours before scanning, the hearts were rinsed and placed in a water bath for rehydration. Data was acquired with a 3T scanner (Philips, Achieva) using an 8-channel head coil with a DW-STE sequence with multi-shot EPI readout. Acquisition parameters were: FOV: 128 × 128 mm2, voxel size: 2 × 2 × 2 mm3, TE/TR: 55/3500 ms, 9 slices (5 mm gap), 14 b-values (250 to 3500 s/mm2), TM: 30, 50, 70, 110, 150, 200, 250 and 300 ms, 8 gradient directions. The data was registered to the average b = 0 images followed by Rician noise suppression. The data was fitted using a single tensor (LLS) and a two-tensor model (NLS). The latter was constrained to follow the fiber directions found in the single tensor model.
We have shown that de DTI derived parameters and fiber tractography depend on both diffusion time and b-value. Furthermore the two-tensor fit shows two compartments with different diffusion properties. Here the compartment with the slower diffusion could be within the muscle fibers where the actin and myosin filaments are tightly packed whereas the faster diffusion compartment could be the space surrounding the sarcomeres. It is important to consider these findings in the interpretation of in-vivo cardiac DTI data.
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