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  • Workshop presentation
  • Open Access

Dynamic tracking of manganese uptake in mouse hearts by rapid multi-slice T1 mapping

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Journal of Cardiovascular Magnetic Resonance201416 (Suppl 1) :W35

https://doi.org/10.1186/1532-429X-16-S1-W35

  • Published:

Keywords

  • Mouse Heart
  • Saturation Recovery
  • Volume Coil
  • Dynamic Tracking
  • Magnetization Recovery

Background

Manganese (Mn2+)-enhanced MRI (MEMRI) has the potential for in vivo assessment of the voltage-gated L-type Ca2+ channel activity. Quantitative assessment of Mn2+ uptake via Ca2+ channels requires fast and accurate T1 mapping. In the current study, a multi-slice saturation recovery Look-Locker (MSRLL) method was developed for T1 mapping of the whole mouse heart in < 3 min.

Methods

MSRLL Sequence

A schematic diagram of the MSRLL pulse sequence is shown in Figure 1. An ECG-triggered saturation module was applied at the beginning of each phase-encoding step, followed by the acquisition of k-space lines along the magnetization recovery curve in multiple slices. ECG-triggered image acquisition was performed at late diastole.
Figure 1
Figure 1

MSRLL pulse sequence. After 90° non-slice selective saturation pulses, 10 sequential multi-slice FLASH acquisitions separated by an interval τ were applied. Each block on the relaxation curve represents a multi-slice acquisition module.

Phantom Study

All MRI studies were performed on a horizontal 7.0T Bruker scanner with a 35 mm volume coil. The MSRLL method was first validated in vitro using a multi-compartment phantom with MnCl2 solution ranging from 30 μM to 1000 μM. T1 maps of 5 slices were compared with those acquired with a previously validated single-slice method (SRLL). Imaging parameters were: flip angle, 10°; TE, 1.9 msec; slice thickness, 1 mm; number of average, 1; field of view, 3 × 3 cm2; matrix size, 128 × 64.

In Vivo Study

In vivo MEMRI studies were performed in 3-month-old FVB mice (n = 19). T1 maps of three adjacent short-axis slices at mid-ventricular levels were acquired with the same imaging parameters as those used in vitro. Continuous T1 mapping was performed during the 30 min of MnCl2 infusion through tail vein (0.2 mL/hr) and the 15 minutes washout. To investigate the Mn2+-induced relaxivity changes, two different MnCl2 solutions at 126 mM (n = 9) and 63 mM (n = 10) were used. Validation study was performed either at baseline (n = 10) or post-contrast (n = 10).

Results

In vitro studies showed strong agreement between MSRLL and SRLL. Average imaging time in vivo was 140~166 s. Shown in Figure 2 are representative T1 maps acquired at baseline (Figure 2a-c) and after Mn2+ infusion (Figure 2d-f). All three slices showed significant reduction in T1 after Mn2+ infusion. The time courses of the R1 changes for all three slices are presented in Figure 2g. In general, higher Mn2+ dose induced larger increase in R1 during Mn2+ infusion.
Figure 2
Figure 2

R 1 changes in dynamic MEMRI study. a-c. Pre-contrast T1 maps of the three slices. d-f. Post-contrast T1 maps of the three slices. g. Time courses of R1 changes.

Conclusions

An ECG-triggered, multi-slice saturation-recovery Look-Locker method was developed for fast and complete cardiac T1 mapping in mice. Validity and utility of this method was well demonstrated in the phantom and in vivo two-dose MEMRI studies.

Funding

This study was supported by NIH Grants HL73315 and HL86935 (Yu).

Authors’ Affiliations

(1)
Biomedical Engineering, Case Western Reserve University, Cleveland, Ohio, USA

Copyright

© Jiang and Yu; licensee BioMed Central Ltd. 2014

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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