Skip to content

Advertisement

  • Moderated poster presentation
  • Open Access

Ischemic postconditioning diminishes intramyocardial hemorrhage in acute reperfused myocardial infarction in rats, evaluated by CMR at 7T

  • 1,
  • 1,
  • 1 and
  • 1
Journal of Cardiovascular Magnetic Resonance201517 (Suppl 1) :M5

https://doi.org/10.1186/1532-429X-17-S1-M5

  • Published:

Keywords

  • Acute Myocardial Infarction
  • Infarct Size
  • Cardiac Magnetic Resonance
  • Acute Myocardial Infarction
  • Late Gadolinium Enhancement

Background

Ischemic postconditioning (IPOC) is considered to be a beneficial tool which can reduce reperfusion injury in acute myocardial infarction (AMI). We aim to assess the effect of IPOC on intramyocardial hemorrhage in rats with AMI.

Methods

Twenty Sprague-Dawley rats (female, 250-300g) were divided into two groups: control group and IPOC group. The AMI model was built by ligating the left anterior descending coronary artery. All rats were subjected to 60 min ischemia, after that control group received direct reperfusion and IPOC group received 1 min of postconditioning (3 cycles of 10s reperfusion and 10s reocclusion) before permanent reperfusion. 48 hours later, all the rats were underwent cardiac magnetic resonance imaging (CMR) at 7.0T (Bruker BioSpect70/30, Germany). Cine (TR/TE=5.2ms/2.0ms, FA=10°, MTX=192×192, FOV=50×50mm, slice thickness=1.5mm), Late gadolinium enhancement (TR/TE=5.2ms/1.8ms, FA=25°, MTX=256×256, FOV=50×50mm, slice thickness=1.5mm) and simplified T2-mapping (TR/TE=1500ms/10,20,30ms, MTX=192×192, FOV=50×50mm, slice thickness=1.5mm) were performed to acquire cardiac function indexes(EDV, ESV and EF), infarct size (LV%) and the area at risk (edematous area, LV%) respectively. All rats were sacrificed after CMR and the hearts were excised for hematoxylin-eosin staining. The results were expressed as mean ± SD and independent sample T-test was performed to determine difference between groups.

Results

Sixteen rats left (Control=9; IPOC=7) for three rats were died during operation and one was excluded for image artifacts. Intramyocardial hemorrhage size was significantly larger in control group compared with IPOC group(9.3±2.6% vs. 2.4±0.3%, P = 0.00).There was no significant difference was observed in infarct size (46.7±13.1% vs. 37.7±12.1%, P =NS), area at risk (65±11.8% vs. 51.4±13.2%, P = NS) and EF(57.7±12.4% vs. 57.6±13.5%, P = NS). Figure 1
Figure 1
Figure 1

Intromyocardial hemorrhage in control group and IPOC group.

Conclusions

IPOC may significantly reduce intramyocardial hemorrhage but cannot decrease myocardial edema and myocardial infarct size and it all failed to improve EF. It is known that intramyocardial hemorrhage is included as an aspect of reperfusion injury, so we can conclude that IPOC has a positive effect to dimimish reperfusion injury.

Funding

This study was supported by The National Natural Science Foundation of China (81130027).

Authors’ Affiliations

(1)
West China Hospital, Sichuan University, Chengdu, China

Copyright

© Zhang et al; licensee BioMed Central Ltd. 2015

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

Advertisement